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    公开号:AU2008231441A1
    申请号:U2008231441
    申请日:2008-03-25
    公开日:2008-10-02
    发明作者:Hamed Aissaoui；Christoph Boss；Markus Gude；Ralf Koberstein；Thierry Sifferlen
    申请人:Actelion Pharmaceuticals Ltd；
    IPC主号:C07D277-04
    专利说明:
    WO 2008/117241 PCT/IB2008/051110 Thiazolidine derivatives The present invention relates to novel thiazolidine derivatives of formula (1) and their use as pharmaceuticals. The invention also concerns related aspects including 5 processes for the preparation of the compounds, pharmaceutical compositions containing one or more compounds of formula (1), and especially their use as orexin receptor antagonists. Orexins (orexin A or OX-A and orexin B or OX-B) are novel neuropeptides found in 1998 by two research groups, orexin A is a 33 amino acid peptide and orexin B is a 28 10 amino acid peptide (Sakurai T. etal., Cell, 1998, 92, 573-585). Orexins are produced in discrete neurons of the lateral hypothalamus and bind to G-protein-coupled receptors (OX, and OX 2 receptors). The orexin-1 receptor (OX 1 ) is selective for OX-A, and the orexin-2 receptor (OX 2 ) is capable to bind OX-A as well as OX-B. Orexins are found to stimulate food consumption in rats suggesting a physiological role for these peptides as 15 mediators in the central feedback mechanism that regulates feeding behaviour (Sakurai T. et al., Cell, 1998, 92, 573-585). On the other hand, it was also observed that orexins regulate states of sleep and wakefulness opening potentially novel therapeutic approaches to narcolepsy as well as insomnia and other sleep disorders (Chemelli R.M. et al., Cell, 1999, 98, 437-451). Orexin receptors are found in the 20 mammalian brain and may have numerous implications in pathologies as known from the literature. The present invention provides thiazolidine derivatives, which are non-peptide antagonists of human orexin receptors. These compounds are in particular of potential use in the treatment of e.g. eating disorders, drinking disorders, sleep disorders, or 25 cognitive dysfunctions in psychiatric and neurologic disorders. Up to now, several low molecular weight compounds are known having a potential to antagonise either specifically OX, or OX 2 , or both receptors at the same time. Piperidine derivatives useful as orexin receptor antagonists are disclosed in WOO1/96302. Morpholine derivatives useful as orexin receptor antagonists are 30 disclosed in WO02/44172. N-Aroyl cyclic amine derivatives useful as orexin receptor antagonists are disclosed in WO02/90355. The present invention describes for the first time thiazolidine derivatives as orexin receptor antagonists.
    WO 2008/117241 PCT/IB2008/051110 2 i) A first aspect of the invention consists of a compound of the formula (1) S : H <N N R A O (1) wherein 5 A represents _N S
    R
    2 / D D ,or D X represents 0, or S;
    R
    2 represents (C_4)alkyl; D represents aryl, which is unsubstituted, mono-, di, or tri-substituted wherein the 10 substituents are independently selected from the group consisting of (C_4)alkyl, (C_4)alkoxy, trifluoromethyl, and halogen;
    R
    1 represents aryl, wherein the aryl group is selected from the group consisting of a phenyl-, a naphthyl-, a 2,3-dihydro-benzofuranyl-, a benzo[1,3]dioxolyl-, a 2,3-dihydro benzo[1,4]dioxinyl-, a 4H-benzo[1,3]dioxinyl, a 2H-chromenyl-, a chromanyl-, a 3,4 15 dihydro-2H-benzo[1,4]oxazinyl-, and a 3-biphenyl group, wherein said groups are unsubstituted, mono-, di, or tri-substituted wherein the substituents are independently selected from the group consisting of (C_4)alkyl, (C_4)alkoxy, trifluoromethyl, halogen and nitro; or R 1 represents heteroaryl, which is unsubstituted, mono-, di, or tri-substituted, 20 wherein the substituents are independently selected from the group consisting of (C, 4 )alkyl, (C_4)alkoxy, halogen, hydroxy-(C_4)alkyl, and trifluoromethyl. The term "halogen" means fluorine, chlorine, or bromine, preferably fluorine or chlorine. The term "(C_4)alkyl" means a straight-chain or branched-chain alkyl group with 1 to 4 carbon atoms. Examples of (C_ 4 )alkyl groups are methyl, ethyl, propyl, isopropyl, 25 n-butyl, isobutyl, sec.-butyl and tert.-butyl. Preferred are methyl and ethyl. Most preferred is methyl.
    WO 2008/117241 PCT/IB2008/051110 3 The term "(C_4)alkoxy" means a group of the formula (C_4)alkyl-O- in which the term "(C_4)alkyl" has the previously given significance. Examples of (C_4)alkoxy groups are methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy, isobutoxy, sec.-butoxy and tert.
    butoxy. Preferred are methoxy and ethoxy. Most preferred is methoxy. 5 "D" representing "aryl" means unsubstituted, mono-, di-, or tri-substituted naphthyl or (preferably) phenyl (preferred mono- or di-substituted phenyl), wherein the substituents are independently selected from the group consisting of (C,_ 4 )alkyl, (C,_ 4 )alkoxy, trifluoromethyl, and halogen; most preferably from (C_ 4 )alkyl, (C_ 4 )alkoxy and halogen. Examples of "D" representing "aryl" are phenyl, 3-methylphenyl, 4-methylphenyl, 3,4 10 dimethylphenyl, 2-methoxyphenyl, 3-methoxyphenyl, 4-methoxyphenyl, 2-fluorophenyl, 3-fluorophenyl, 4-fluorophenyl, 3,4-difluorophenyl, 3,4-dichlorophenyl, 3 trifluoromethylphenyl and 4-trifluoromethylphenyl. "R'" representing "aryl" means a group selected from the group consisting of a phenyl, a naphthyl, a 2,3-dihydro-benzofuranyl-, a benzo[1,3]dioxolyl-, a 2,3-dihydro 15 benzo[1,4]dioxinyl-, a 4H-benzo[1,3]dioxinyl, a 2H-chromenyl-, a chromanyl-, or a 3,4 dihydro-2H-benzo[1,4]oxazinyl, and a 3-biphenyl-group. The above mentioned aryl group as used for "R'" is unsubstituted, mono-, di-, or tri-substituted wherein the substituents are independently selected from the group consisting of (C_4)alkyl, (C, 4 )alkoxy, trifluoromethyl, halogen and nitro; preferably from (C_4)alkyl, (C_4)alkoxy, 20 trifluoromethyl, and halogen; most preferably from (C_ 4 )alkyl, (C_ 4 )alkoxy and halogen. In one sub-embodiment, "R'" representing "aryl" means a naphthyl- or (preferably) a phenyl group, which group is unsubstituted, mono-, di-, or tri-substituted (preferred: monosubstituted), wherein the substituents are independently selected from the group consisting of (C_4)alkyl, (C_4)alkoxy, trifluoromethyl, halogen and nitro; especially from 25 (C_4)alkyl, (C_4)alkoxy, halogen, and trifluoromethyl (preferred: halogen). Additionally, in another sub-embodiment "R'" representing "aryl" means a 2,3-dihydro-benzofuranyl ; a benzo[1,3]dioxolyl-; a 2,3-dihydro-benzo[1,4]dioxinyl-; a 4H-benzo[1,3]dioxinyl-, a 2H-chromenyl-, a chromanyl-, or a 3,4-dihydro-2H-benzo[1,4]oxazinyl group (especially a 2,3-dihydro-benzofuranyl-, a benzo[1,3]dioxolyl-, a 2,3-dihydro-benzo[1,4]dioxinyl-, or 30 a 4H-benzo[1,3]dioxinyl group). Said aryl groups of this sub-embodiment are unsubstituted, mono-, di-, or tri-substituted (preferred unsubstituted, mono- or di substituted) wherein the substituents are independently selected from the group consisting of (C_4)alkyl, (C_4)alkoxy, trifluoromethyl, and halogen; preferred from
    (C_
    4 )alkyl, (C_ 4 )alkoxy and halogen. In a preferred sub-embodiment, 2,3-dihydro 35 benzofuranyl-, benzo[1,3]dioxolyl-, 2,3-dihydro-benzo[1,4]dioxinyl-, 4H- WO 2008/117241 PCT/IB2008/051110 4 benzo[1,3]dioxinyl-, 2H-chromenyl-, chromanyl-, and 3-biphenyl groups are preferably unsubstituted. 3,4-Dihydro-2H-benzo[1,4]oxazinyl groups are preferably unsubstituted or mono-substituted with (C 1 .4)alkyl (especially methyl). In another preferred sub embodiment, a 2,3-dihydro-benzofuranyl group may also be disubstituted, wherein the 5 substituents are independently selected from halogen and (C_ 4 )alkoxy. Examples of R 1 representing "aryl" are naphthyl, 3-bromophenyl, 3-nitrophenyl, 3 biphenyl, 2,3-dihydro-benzofuran-4-yl, 2,3-dihydro-benzofuran-7-yl, 7-chloro-2 methoxy-2,3-dihydro-benzofuran-4-yl, 4H-benzo[1,3]dioxin-5-yl, 4H-benzo[1,3]dioxin-8 yl, benzo[1,3]dioxol-4-yl, 2,3-dihydro-benzo[1,4]dioxin-5-yl, chromen-5-yl, chroman-5 10 yl, chroman-8-yl, 3,4-dihydro-2H-benzo[1,4]oxazin-5-yl, 4-methyl-3,4-dihydro-2H benzo[1,4]oxazine-5-yl, 3,4-dihydro-2H-benzo[1,4]oxazin-8-yl, and 4-methyl-3,4 dihydro-2H-benzo[1,4]oxazine-8-yl. Preferred are 2,3-dihydro-benzofuran-4-yl, 2,3 dihydro-benzofuran-7-yl, 7-chloro-2-methoxy-2,3-dihydro-benzofuran-4-yl, 2,3-dihydro benzo[1,4]dioxin-5-yl, chroman-5-yl, chroman-8-yl, 3,4-dihydro-2H-benzo[1,4]oxazin-5 15 yl, 4-methyl-3,4-dihydro-2H-benzo[1,4]oxazine-5-yl, 3,4-dihydro-2H-benzo[1,4]oxazin 8-yl, and 4-methyl-3,4-dihydro-2H-benzo[1,4]oxazine-8-yl. In another embodiment, preferred examples of R 1 representing "aryl" are 2,3-dihydro-benzo[1,4]dioxinyl and 3-bromophenyl. The term "heteroaryl" means a 5- to 10-membered monocyclic or bicyclic (preferred 8 20 to 9-membered bicyclic) aromatic ring containing 1, 2 or 3 heteroatoms, each independently selected from oxygen, nitrogen and sulfur. Examples of such heteroaryl groups are furanyl, oxazolyl, isoxazolyl, oxadiazolyl, thienyl, thiazolyl, isothiazolyl, thiadiazolyl, pyrrolyl, imidazolyl, pyrazolyl, triazolyl, pyridyl, pyrimidyl, pyridazinyl, pyrazinyl, indolyl, isoindolyl, benzofuranyl, isobenzofuranyl, benzothiophenyl, indazolyl, 25 benzimidazolyl, benzoxazolyl, benzisoxazolyl, benzothiazolyl, benzotriazolyl, benzoxadiazolyl, benzothiadiazolyl, quinolinyl, isoquinolinyl, naphthyridinyl, cinnolinyl, quinazolinyl, quinoxalinyl, phthalazinyl, pyrazolo[1,5-a]pyridyl, pyrazolo[1,5-a]pyrimidyl, imidazo[1,2-a]pyridyl and imidazo[2,1-b]thiazolyl. In addition to the above list, further examples are benzoisothiazolyl, and pyrrolo[2,1-b]thiazolyl. Preferred examples are 30 thienyl, thiazolyl, pyrazolyl, pyridyl, indolyl, benzofuranyl, indazolyl, benzoxazolyl, benzisoxazolyl, benzothiazolyl, benzoxadiazolyl, benzothiadiazolyl, imidazo[1,2 a]pyridyl, imidazo[2,1-b]thiazolyl, benzoisothiazolyl, and pyrrolo[2,1-b]thiazolyl. In another embodiment, preferred examples are benzisoxazolyl, benzoxadiazolyl, benzothiadiazolyl, imidazo[1,2-a]pyridyl, imidazo[2,1-b]thiazolyl, benzoisothiazolyl, and 35 pyrrolo[2,1-b]thiazolyl. The above-mentioned heteroaryl groups are unsubstituted, WO 2008/117241 PCT/IB2008/051110 5 mono-, di-, or tri-substituted (preferred unsubstituted, mono-, or di-substituted) wherein the substituents are independently selected from the group consisting of (C_4)alkyl, (C, 4 )alkoxy, halogen, hydroxy-(C_4)alkyl, and trifluoromethyl; especially from (C_4)alkyl, (C_4)alkoxy, halogen, and trifluoromethyl; preferred from (C_4)alkyl, halogen, and 5 trifluoromethyl. In another embodiment, preferred examples of such heteroaryl groups are thienyl, pyridyl, indolyl, indazolyl, benzofuranyl, and imidazo[2,1-b]thiazolyl, which groups may be unsubstituted, mono-, di-, or tri-substituted (preferred unsubstituted, mono-, or di-substituted, most preferred unsubstituted, or mono-substituted) wherein the substituents are independently selected from the group consisting of (C_4)alkyl, (C, 10 4 )alkoxy, halogen, and trifluoromethyl (preferred (C4)alkyl, and halogen). In particular, the above mentioned "heteroaryl" groups as used for the substituent "R 1 " are preferably substituted as follows: thienyl groups are substituted with halogen; thiazolyl groups are di-substituted with (C_4)alkyl; pyrazolyl groups are di-substituted with (C4)alkyl; pyridyl groups are mono-substituted with halogen; indolyl groups are 15 mono-substituted with (C_4)alkyl (especially methyl); indazolyl groups are unsubstituted or mono-substituted with (C_ 4 )alkyl (especially methyl); benzoxazolyl groups are unsubstituted, or mono-substituted with (C_4)alkyl (especially methyl); benzothiazolyl groups are unsubstituted or mono-substituted with halogen; benzisoxazolyl, benzoxadiazolyl, benzothiadiazolyl, and benzoisothiazolyl groups are unsubstituted; 20 imidazo[1,2-a]pyridyl are unsubstituted or mono-substituted with (C_4)alkyl (especially methyl); pyrrolo[2,1-b]thiazolyl groups are unsubstituted or mono-substituted with (C,_ 4 )alkyl (especially methyl); and imidazo[2,1-b] thiazolyl groups are mono-substituted with (C,_4)alkyl (especially methyl); benzofuranyl groups are preferably unsubstituted, mono-, or di-substituted wherein the substituents are independently selected from (C, 25 4 )alkyl, halogen, hydroxy-(C_4)alkyl, and trifluoromethyl, especially from (C_4)alkyl, halogen, and trifluoromethyl. Examples of said benzofuranyl groups are benzofuran-4 yl, 7-chloro-benzofuran-4-yl, 7-fluoro-benzofuran-4-yl, 2-fluoro-benzofuran-4-yl, 3 methyl-benzofuran-4-yl, 2-methyl-benzofuran-4-yl, 2-hydroxymethyl-benzofuran-4-yl, 5 chloro-2-methyl-benzofuran-4-yl, 7-chloro-2-methyl-benzofuran-4-yl, 7-fluoro-2-methyl 30 benzofuran-4-yl, 6-ch loro-2-methyl-benzofu ran-4-yl, 6-fluoro-2-methyl-benzofuran-4-yl, 2,3-dimethyl-benzofuran-4-yl, 2-trifl uoromethyl-benzofuran-4-yl, 7-trifluoromethyl benzofuran-4-yl, 2-methyl-6-trifluoromethyl-benzofuran-4-yl, and 2-methyl-7 trifluoromethyl-benzofuran-4-yl.
    WO 2008/117241 PCT/IB2008/051110 6 ii) A further embodiment of the invention relates to thiazolidine derivatives of formula (1) according to embodiment i), wherein the stereogenic center at the thiazolidine ring is in (R)-configuration. iii) A further embodiment of the invention relates to thiazolidine derivatives according to 5 any one of embodiments i) or ii), wherein A represents R 2 R2 S N N Dor D iv) A further embodiment of the invention relates to thiazolidine derivatives according to any one of embodiments i) to iii), wherein A represents
    R
    2 / 10 X D v) A further embodiment of the invention relates to thiazolidine derivatives according to any one of embodiments i) to iv), wherein X represents S. vi) A further embodiment of the invention relates to thiazolidine derivatives according to 15 any one of embodiments i) to iv), wherein X represents 0. vii) A further embodiment of the invention relates to thiazolidine derivatives according to any one of embodiments i) to vi), wherein
    R
    2 represents methyl. 20 viii) A further embodiment of the invention relates to thiazolidine derivatives according to embodiments i) or ii), wherein A represents D ix) A further embodiment of the invention relates to thiazolidine derivatives according to 25 any one of embodiments i) to viii), wherein D represents unsubstituted, mono-, di-, or tri-substituted phenyl, wherein the substituents are independently selected from the group consisting of (C)alkyl, (C)alkoxy, trifluoromethyl, and halogen.
    WO 2008/117241 PCT/IB2008/051110 7 x) A further embodiment of the invention relates to thiazolidine derivatives according to any one of embodiments i) to ix), wherein
    R
    1 represents aryl, wherein the aryl group is selected from the group consisting of a 2,3-dihydro-benzofuranyl-, a benzo[1,3]dioxolyl-, a 2,3-dihydro-benzo[1,4]dioxinyl-, a 5 4H-benzo[1,3]dioxinyl, a 2H-chromenyl-, a chromanyl-, and a 3,4-dihydro-2H benzo[1,4]oxazinyl group, wherein said groups are unsubstituted, mono-, or di substituted wherein the substituents are independently selected from the group consisting of (C_4)alkyl, (C_4)alkoxy and halogen; or R 1 represents heteroaryl, which is unsubstituted, mono-, di, or tri-substituted, 10 wherein the substituents are independently selected from the group consisting of (C, 4 )alkyl, (C_4)alkoxy, halogen, hydroxy-(C_4)alkyl, and trifluoromethyl. xi) A further embodiment of the invention relates to thiazolidine derivatives according to any one of embodiments i) to x), wherein R 1 represents heteroaryl, wherein said hetereroaryl is selected from the group consisting of thienyl, thiazolyl, pyrazolyl, pyridyl, 15 indolyl, benzofuranyl, indazolyl, benzoxazolyl, benzisoxazolyl, benzothiazolyl, benzoxadiazolyl, benzothiadiazolyl, imidazo[1,2-a]pyridyl, imidazo[2,1 -b]thiazolyl, benzoisothiazolyl, and pyrrolo[2,1-b]thiazolyl, wherein said groups are unsubstituted, mono-, di-, or tri-substituted (preferred unsubstituted, mono-, or di-substituted), wherein the substituents are independently selected from the group consisting of (C_4)alkyl, (C, 20 4 )alkoxy, halogen, hydroxy-(C_4)alkyl, and trifluoromethyl. xii) A further embodiment of the invention relates to thiazolidine derivatives according to any one of embodiments i) to x), wherein R 1 represents heteroaryl, wherein said hetereroaryl is selected from the group consisting of benzisoxazolyl, benzoxadiazolyl, benzothiadiazolyl, imidazo[1,2-a]pyridyl, imidazo[2,1-b]thiazolyl, benzoisothiazolyl, and 25 pyrrolo[2,1-b]thiazolyl, wherein said groups are unsubstituted, mono-, or di-substituted wherein the substituents are independently selected from the group consisting of (C_4)alkyl, (C_4)alkoxy, halogen, and trifluoromethyl. xiii) A further embodiment of the invention relates to thiazolidine derivatives according to any one of embodiments i) to xi), wherein, in case R 1 represents heteroaryl, it 30 represents a group selected from WO 2008/117241 PCT/IB2008/051110 8 / N' )tNj sl NN N: N_6N'/- S6 _<S:6 N'_6 S_ N CI NN N S NN O S N0N X N -:1 0 1M and benzofuranyl which is unsubstituted, mono, or di-substituted wherein the substituents are independently selected from (C_4)alkyl, halogen, hydroxy-(C_4)alkyl, and trifluoromethyl. 5 xiv) A further embodiment of the invention relates to thiazolidine derivatives according to any one of embodiments i) to xi), wherein R 1 represents heteroaryl, wherein said hetereroaryl is selected from the group consisting of thienyl, pyridyl, indolyl, benzofuranyl, indazolyl, and imidazo[2,1-b]thiazolyl, wherein said groups are unsubstituted, mono-, di-, or tri-substituted wherein the substituents are independently 10 selected from the group consisting of (C_4)alkyl, (C_4)alkoxy, halogen, and trifluoromethyl. xv) A further embodiment of the invention relates to thiazolidine derivatives according to any one of embodiments i) to ix), wherein R 1 represents a naphthyl- or a phenyl group (preferred) which is unsubstituted, mono-, di-, or tri-substituted (preferred: mono 15 substituted), wherein the substituents are independently selected from the group consisting of (C_4)alkyl, (C_4)alkoxy, trifluoromethyl, halogen and nitro (especially from (C_4)alkyl, (C_4)alkoxy, halogen, and trifluoromethyl (preferred: halogen)). xvi) A further embodiment of the invention relates to thiazolidine derivatives according to any one of embodiments i) to x), wherein 20 R 1 represents aryl, wherein the aryl group is selected from the group consisting of a 2,3-dihydro-benzofuranyl-, a benzo[1,3]dioxolyl-, a 2,3-dihydro-benzo[1,4]dioxinyl-, a 4H-benzo[1,3]dioxinyl, a 2H-chromenyl-, a chromanyl-, and a 3,4-dihydro-2H benzo[1,4]oxazinyl group, wherein said groups are unsubstituted, mono-, or di- WO 2008/117241 PCT/IB2008/051110 9 substituted wherein the substituents are independently selected from the group consisting of (C_4)alkyl, (C_4)alkoxy and halogen. xvii) A further embodiment of the invention relates to thiazolidine derivatives according to any one of embodiments i) to x) or xvi), wherein, in case R 1 represents an aryl group, 5 said aryl group is selected from the group consisting of a 2,3-dihydro-benzofuranyl-, a 2,3-dihydro-benzo[1,4]dioxinyl-, a chromanyl-, and a 3,4-dihydro-2H-benzo[1,4]oxazinyl group, wherein said groups are unsubstituted, mono-, or di-substituted wherein the substituents are independently selected from the group consisting of (C_4)alkyl, (C, 4 )alkoxy and halogen. 10 xviii) A further embodiment of the invention relates to thiazolidine derivatives according to any one of embodiments i) to x), or xvi), wherein, in case R 1 represents an aryl group, said aryl group is selected from the group consisting of a 2,3-dihydro benzofuranyl-, a benzo[1,3]dioxolyl-, a 2,3-dihydro-benzo[1,4]dioxinyl-, or a 4H benzo[1,3]dioxinyl group, wherein said groups are unsubstituted. 15 xix) A further embodiment of the invention relates to thiazolidine derivatives according to any one of embodiments i) to xi), wherein R 1 represents a group selected from N' NN N N N N N /> S/- s ,N N IN and . xx) A further embodiment of the invention relates to thiazolidine derivatives according to any one of embodiments i) to xi), wherein R 1 represents a group selected from N 20 and . xxi) A further embodiment of the invention relates to thiazolidine derivatives according to any one of embodiments i) to xi), wherein R 1 represents a group selected from WO 2008/117241 PCT/IB2008/051110 10 S N N N />/ >__ N ,N , and N . xxii) A further embodiment of the invention relates to thiazolidine derivatives according to any one of embodiments i) to xi) or xxi), wherein R 1 represents N 5 xxiii) A further embodiment of the invention relates to thiazolidine derivatives of formula (1) according to embodiments i) or ii) wherein A represents N DS D D N D,or D; X represents S, or 0; 10 D represents unsubstituted, mono-, or di-substituted phenyl, wherein the substituents are independently selected from the group consisting of (C_ 4 )alkyl, (C_ 4 )alkoxy, trifluoromethyl, and halogen; and
    R
    1 represents a group selected from 2,3-dihydro-benzofuran-4-yl, 2,3-dihydro benzofuran-7-yl, 7-chloro-2-methoxy-2,3-dihydro-benzofuran-4-yl, 2,3-dihydro 15 benzo[1,4]dioxin-5-yl, chroman-5-yl, chroman-8-yl, 3,4-dihydro-2H-benzo[1,4]oxazin-5 yl, 4-methyl-3,4-dihydro-2H-benzo[1,4]oxazine-5-yl, 3,4-dihydro-2H-benzo[1,4]oxazin 8-yl, and 4-methyl-3,4-dihydro-2H-benzo[1,4]oxazine-8-yl; or R 1 represents a group selected from WO 2008/117241 PCT/IB2008/051110 11 / N' )tNj sl NN NN N_6N'/- S6 _<S:6 N'_6 S_ N CI NN N S NN O S N N X N :1N 1iN and benzofuranyl which is unsubstituted, mono, or di-substituted wherein the substituents are independently selected from (C_4)alkyl, halogen, hydroxy-(C_4)alkyl, and trifluoromethyl; 5 or R 1 represents phenyl which is mono-substituted with halogen or nitro, naphthyl, pyridyl which is mono-substituted with halogen, thienyl which is mono-substituted with halogen, thiazolyl which is disubstituted with (C 4)alkyl, or pyrazolyl which is disubstituted with (C_ 4 )alkyl (especially R 1 represents 10S N phenyl which is mono-substituted with halogen, pyridyl which is mono-substituted with halogen, or thienyl which is mono-substituted with halogen). The compounds of formula (1) may contain one or more stereogenic or asymmetric centers, such as one or more asymmetric carbon atoms. Substituents at a double bond 15 may be present in the Z- or E-configuration unless indicated otherwise. The compounds of formula (1) may thus be present as mixtures of stereoisomers or preferably as pure stereoisomers. Mixtures of stereoisomers may be separated in a manner known to a person skilled in the art. Where the plural form is used for compounds, salts, pharmaceutical compositions, 20 diseases and the like, this is intended to mean also a single compound, salt, or the like.
    WO 2008/117241 PCT/IB2008/051110 12 Any reference to a compound of formula (1) is to be understood as referring also to the salts (and especially the pharmaceutically acceptable salts) of such compounds, as appropriate and expedient. The term "pharmaceutically acceptable salts" refers to non-toxic, inorganic or organic 5 acid and/or base addition salts. Reference can be made to "Salt selection for basic drugs", Int. J. Pharm. (1986), 33, 201-217. Examples of preferred compounds of formula (1) according to embodiment i) are selected from the group consisting of: Benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4-carbonyl] 10 thiazolidin-4-ylmethyl}-amide; Benzofuran-4-carboxylic acid {(R)-3-[5-(2-fluoro-phenyl)-2-methyl-thiazole-4-carbonyl] thiazolidin-4-ylmethyl}-amide; Benzofuran-4-carboxylic acid {(R)-3-[5-(3-methoxy-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; 15 Benzofuran-4-carboxylic acid {(R)-3-[2-methyl-5-(3-trifluoromethyl-phenyl)-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; Benzofuran-4-carboxylic acid {(R)-3-[5-(3,4-difluoro-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; Benzofuran-4-carboxylic acid {(R)-3-[5-(2-methoxy-phenyl)-2-methyl-thiazole-4 20 carbonyl]-thiazolidin-4-ylmethyl}-amide; Benzofuran-4-carboxylic acid {(R)-3-[2-methyl-5-(3-trifluoromethyl-phenyl)-oxazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; Benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-p-tolyl-oxazole-4-carbonyl)-thiazolidin 4-ylmethyl]-amide; 25 Benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-oxazole-4-carbonyl] thiazolidin-4-ylmethyl}-amide; Benzofuran-4-carboxylic acid [(R)-3-(2-methyl-4-p-tolyl-thiazole-5-carbonyl)-thiazolidin 4-ylmethyl]-amide; Benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-oxazole-4-carbonyl)-thiazolidin 30 4-ylmethyl]-amide; Benzofuran-4-carboxylic acid {(R)-3-[5-(3,4-dichloro-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; WO 2008/117241 PCT/1B2008/051 110 13 Benzofu ran-4-carboxylic acid {(R)-3-[5-(4-methoxy-phenyl)-2-methyl-oxazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; Benzofu ran-4-carboxylic acid [(R)-3-(4'-methyl-biphenyl-2-carbonyl)-th jazolidi n-4 ylmethyl]-amide; 5 Benzofuran-4-carboxylic acid [(R)-3-(3',4'-dimethyl-biphenyl-2-carbonyl)-thiazolidin-4 ylmethyl]-amide; Benzofuran-4-carboxylic acid [(R)-3-(3'-methyl-biphenyl-2-carbonyl)-thiazolidin-4 ylmethyl]-amide; Benzofu ran-4-carboxylic acid [(R)-3-(3'-methoxy-biphenyl-2-carbonyl)-th iazolidin-4 10 ylmethyl]-amide; Benzofuran-4-carboxylic acid [(R)-3-(4'-fluoro-biphenyl-2-carbonyl)-thiazolidin-4 ylmethyl]-amide; Benzofu ran-4-carboxylic acid [(R)-3-(4'-methoxy-biphenyl-2-carbonyl)-th iazolidin-4 ylmethyl]-amide; 15 2,3-Dihydro-benzo[1 ,4]dioxine-5-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 1 -Methyl-i H-indazole-3-carboxylic acid {(R) -3- [5-(4-flu oro-phe nyl)-2-m ethyl -th iazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; Benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-phenyl-thiazole-4-carbonyl)-thiazolidin 20 4-yl methyl] -am ide; 1 -Methyl-i1 H-indazole-3-carboxylic acid [(R)-3-(2-methyl-5-phenyl-thiazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; 1 -Methyl-i H-indazole-3-carboxylic acid [(R)-3-(2-methyl-5-p-tolyl-thiazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; 25 1 -Methyl-i H-indazole-3-carboxylic acid {(R)-3-[2-methyl-5-(4-trifluoromethyl-phenyl) thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 1 -Methyl-i H-indazole-3-carboxylic acid {(R) -3- [5-(3-flu oro-phe nyl)-2-m ethyl -th iazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; 1 -Methyl-i H-indazole-3-carboxylic acid {(R)-3-[5-(3,4-dimethyl-phenyl)-2-methyl 30 thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 1 -Methyl-i H-indazole-3-carboxylic acid {(R) -3- [5-(2-flu oro-phe nyl)-2-m ethyl -th iazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; 1 -Methyl-i H-indazole-3-carboxylic acid {(R)-3-[5-(3-methoxy-phenyl)-2-methyl-thiazole 4-carbonyl]-thiazolidin-4-ylmethyl}-amide; WO 2008/117241 PCT/IB2008/051110 14 1-Methyl-1 H-indazole-3-carboxylic acid {(R)-3-[2-methyl-5-(3-trifluoromethyl-phenyl) thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 1-Methyl-1 H-indazole-3-carboxylic acid {(R)-3-[5-(3,4-difluoro-phenyl)-2-methyl thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 5 Benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-p-tolyl-thiazole-4-carbonyl)-thiazolidin 4-ylmethyl]-amide; 1-Methyl-1 H-indazole-3-carboxylic acid {(R)-3-[5-(2-methoxy-phenyl)-2-methyl-thiazole 4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 1-Methyl-1 H-indazole-3-carboxylic acid {(R)-3-[2-methyl-5-(3-trifluoromethyl-phenyl) 10 oxazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 1-Methyl-1 H-indazole-3-carboxylic acid [(R)-3-(2-methyl-5-p-tolyl-oxazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; 1-Methyl-1 H-indazole-3-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-oxazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; 15 1-Methyl-1 H-indazole-3-carboxylic acid [(R)-3-(2-methyl-4-p-tolyl-thiazole-5-carbonyl) thiazolidin-4-ylmethyl]-amide; 1-Methyl-1 H-indazole-3-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-oxazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; 1-Methyl-1 H-indazole-3-carboxylic acid {(R)-3-[5-(3,4-dichloro-phenyl)-2-methyl 20 thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 1-Methyl-1 H-indazole-3-carboxylic acid {(R)-3-[5-(4-methoxy-phenyl)-2-methyl-oxazole 4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 1-Methyl-1 H-indazole-3-carboxylic acid [(R)-3-(4'-methyl-biphenyl-2-carbonyl) thiazolidin-4-ylmethyl]-amide; 25 1-Methyl-1 H-indazole-3-carboxylic acid [(R)-3-(3',4'-dimethyl-biphenyl-2-carbonyl) thiazolidin-4-ylmethyl]-amide; Benzofuran-4-carboxylic acid {(R)-3-[2-methyl-5-(4-trifluoromethyl-phenyl)-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; 1-Methyl-1 H-indazole-3-carboxylic acid [(R)-3-(3'-methyl-biphenyl-2-carbonyl) 30 thiazolidin-4-ylmethyl]-amide; 1-Methyl-1 H-indazole-3-carboxylic acid [(R)-3-(3'-methoxy-biphenyl-2-carbonyl) thiazolidin-4-ylmethyl]-amide; 1-Methyl-1 H-indazole-3-carboxylic acid [(R)-3-(4'-fluoro-biphenyl-2-carbonyl) thiazolidin-4-ylmethyl]-amide; WO 2008/117241 PCT/IB2008/051110 15 1-Methyl-1 H-indazole-3-carboxylic acid [(R)-3-(4'-methoxy-biphenyl-2-carbonyl) thiazolidin-4-ylmethyl]-amide; 3-Bromo-N-{(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin-4 ylmethyl}-benzamide; 5 1-Methyl-1 H-indole-2-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; 4-Bromo-thiophene-2-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; 6-Bromo-pyridine-2-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4 10 carbonyl]-thiazolidin-4-ylmethyl}-amide; 2,3-Dihydro-benzo[1,4]dioxine-5-carboxylic acid [(R)-3-(biphenyl-2-carbonyl) thiazolidin-4-ylmethyl]-amide; Benzofuran-4-carboxylic acid [(R)-3-(biphenyl-2-carbonyl)-thiazolidin-4-ylmethyl] amide; 15 Benzofuran-4-carboxylic acid {(R)-3-[5-(3-fluoro-phenyl)-2-methyl-thiazole-4-carbonyl] thiazolidin-4-ylmethyl}-amide; N-[(R)-3-(Biphenyl-2-carbonyl)-thiazolidin-4-ylmethyl]-3-bromo-benzamide; 1-Methyl-1 H-indole-2-carboxylic acid [(R)-3-(biphenyl-2-carbonyl)-thiazolidin-4 ylmethyl]-amide; 20 1-Methyl-1 H-indazole-3-carboxylic acid [(R)-3-(biphenyl-2-carbonyl)-thiazolidin-4 ylmethyl]-amide; 4-Bromo-thiophene-2-carboxylic acid [(R)-3-(biphenyl-2-carbonyl)-thiazolidin-4 ylmethyl]-amide; 6-Bromo-pyridine-2-carboxylic acid [(R)-3-(biphenyl-2-carbonyl)-thiazolidin-4-ylmethyl] 25 amide; and Benzofuran-4-carboxylic acid {(R)-3-[5-(3,4-dimethyl-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide. Further examples of preferred compounds of formula (1) according to embodiment i) are selected from the group consisting of: 30 Naphthalene-1 -carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; 5-tert-Butyl-2-methyl-2H-pyrazole-3-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2 methyl-th iazole-4-carbonyl]-th iazolidin-4-ylmethyl}-am ide; WO 2008/117241 PCT/IB2008/051110 16 2,3-Dihydro-benzofuran-7-carboxylic acid {(R)-3[5-(4-fluoro-phenyl)-2-methyl-thiazole 4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 2,4-Dimethyl-thiazole-5-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; 5 N-{(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-3 nitro-benzamide; Benzo[b]thiophene-2-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; 2-Methyl-benzofu ran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl) 10 thiazolidin-4-ylmethyl]-amide; 2-Methyl-benzooxazole-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; Imidazo[1,2-a]pyridine-3-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; 15 2-Methyl-imidazo[1,2-a]pyridine-3-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 2,3-Dimethyl-benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 3,4-Dihydro-2H-benzo[1,4]oxazine-5-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole 20 4-carbonyl)-thiazolidin-4-ylmethyl]-amide; 4-Methyl-3,4-Dihydro-2H-benzo[1,4]oxazine-5-carboxylic acid [(R)-3-(2-methyl-5-m tolyl-thiazole-4-carbonyl)-thiazolidin-4-ylmethyl]-amide; Chroman-5-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4 ylmethyl]-amide; 25 Chroman-8-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4 ylmethyl]-amide; 3,4-Dihydro-2H-benzo[1,4]oxazine-8-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole 4-carbonyl)-thiazolidin-4-ylmethyl]-amide; 4-Methyl-3,4-Dihydro-2H-benzo[1,4]oxazine-8-carboxylic acid [(R)-3-(2-methyl-5-m 30 tolyl-thiazole-4-carbonyl)-thiazolidin-4-ylmethyl]-amide; Benzo[d]isoxazole-3-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; Benzo[d]isothiazole-3-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; WO 2008/117241 PCT/IB2008/051110 17 1-Methyl-1H-indazole-3-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; 2,3-D ihydro-benzofu ran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 5 2,3-Dihydro-benzo[1,4]dioxine-5-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 2-Methyl-benzofu ran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; Imidazo[1,2-a]pyridine-3-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4 10 carbonyl]-thiazolidin-4-ylmethyl}-amide; 2-Methyl-imidazo[1,2-a]pyridine-3-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 2,3-Dimethyl-benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 15 3,4-Dihydro-2H-benzo[1,4]oxazine-5-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2 methyl-th iazole-4-carbonyl]-th iazolidin-4-ylmethyl}-am ide; 4-Methyl-3,4-dihydro-2H-benzo[1,4]oxazine-5-carboxylic acid {(R)-3-[5-(4-fluoro phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; Chroman-5-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4-carbonyl] 20 thiazolidin-4-ylmethyl}-amide; Chroman-8-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4-carbonyl] thiazolidin-4-ylmethyl}-amide; 3,4-Dihydro-2H-benzo[1,4]oxazine-8-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2 methyl-th iazole-4-carbonyl]-th iazolidin-4-ylmethyl}-am ide; 25 4-Methyl-3,4-dihydro-2H-benzo[1,4]oxazine-8-carboxylic acid {(R)-3-[5-(4-fluoro phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 2,3-Dihydro-benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole 4-carbonyl]-thiazolidin-4-ylmethyl}-amide; Benzooxazole-7-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl) 30 thiazolidin-4-ylmethyl]-amide; Benzooxazole-7-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; 2-Methyl-benzooxazole-7-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; WO 2008/117241 PCT/1B2008/051 110 18 2-Methyl-benzooxazole-7-carboxylic acid {( R)-3-[5-(4-fI uoro-phenyl)-2-methyl-thiazole 4-carbonyl]-thiazolidin-4-ylmethyl}-amide; Benzoth iazole-7-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-th iazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; 5 Benzoth iazole-7-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-th iazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; 7-Oh Ioro-benzof uran -4-carboxyl ic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; 7-Oh Ioro-benzof uran -4-carboxyl ic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-th iazole-4 10 carbonyl]-thiazolidin-4-ylmethyl}-amide; 7-Fluoro-benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; 7-Fluoro-benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; 15 Pyrrolo[2, 1-b]thiazole-7-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; Pyrrolo[2, 1-b]thiazole-7-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; 6-Methyl-pyrrolo[2, 1-b]th iazole-7-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-th iazole-4 20 carbonyl)-thiazolidin-4-ylmethyl]-amide; 6-Methyl-pyrrolo[2, 1-b]thiazole-7-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 7-Chloro-2-methoxy-2,3-dihydro-benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-m tolyl-th iazole-4-carbonyl)-thiazolidin-4-ylmethyl]-amide; 25 7-Oh Ioro-2-methoxy-2,3-di hydro-benzof uran -4-carboxyl ic acid {( R)-3-[5-(4-fI uoro phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 2-Oh Ioro-benzothiazole-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 2-Oh Ioro-benzoth iazole-4-carboxylic acid {( R)-3-[5-(4-fI uoro-phenyl)-2-methyl-thiazole 30 4-carbonyl]-thiazolidin-4-ylmethyl}-amide; Benzoth iazole-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-th iazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; Benzoth iazole-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-th iazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; WO 2008/117241 PCT/IB2008/051110 19 Benzo[2,1,3]th iadiazole-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; Benzo[2,1,3]thiadiazole-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole 4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 5 7-Trifl uoromethyl-benzofu ran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 7-Trifl uoromethyl-benzofu ran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 3-Methyl-benzofu ran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl) 10 thiazolidin-4-ylmethyl]-amide; 3-Methyl-benzofu ran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; Benzo[2,1,3]oxadiazole-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 15 Benzo[2,1,3]oxadiazole-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole 4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 2-Hydroxymethyl-benzofu ran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 2-Hydroxymethyl-benzofu ran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl 20 thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 2-Methyl-benzofu ran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; 5-Chloro-2-methyl-benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 25 7-Chloro-2-methyl-benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 7-Fluoro-2-methyl-benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 2-Methyl-7-trifluoromethyl-benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl 30 thiazole-4-carbonyl)-thiazolidin-4-ylmethyl]-amide; 6-Chloro-2-methyl-benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 6-Fluoro-2-methyl-benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; WO 2008/117241 PCT/IB2008/051110 20 2-Methyl-6-trifluoromethyl-benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl thiazole-4-carbonyl)-thiazolidin-4-ylmethyl]-amide; 5-Ch loro-2-methyl-benzofu ran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 5 7-Chloro-2-methyl-benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 7-Fluoro-2-methyl-benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 2-Methyl-7-trifl uoromethyl-benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2 10 methyl-th iazole-4-carbonyl]-th iazolidin-4-ylmethyl}-am ide; 6-Chloro-2-methyl-benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 6-Fluoro-2-methyl-benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 15 2-Methyl-6-trifluoromethyl-benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2 methyl-th iazole-4-carbonyl]-th iazolidin-4-ylmethyl}-am ide; and 2-Trifl uoromethyl-benzofu ran-4-carboxyl ic acid [3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide. Further examples of preferred compounds of formula (1) according to embodiment i) 20 are selected from the group consisting of: 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 6-Methyl-imidazo[2,1-b]thiazole-5-carboxylic acid [(R)-3-(2-methyl-5-phenyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 25 6-Methyl-imidazo[2,1-b]thiazole-5-carboxylic acid [(R)-3-(2-methyl-5-p-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 6-Methyl-imidazo[2,1-b]thiazole-5-carboxylic acid {(R)-3-[2-methyl-5-(4-trifluoromethyl phenyl)-thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid {(R)-3-[5-(3-fluoro-phenyl)-2-methyl 30 thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid {(R)-3-[5-(3,4-dimethyl-phenyl)-2 methyl-th iazole-4-carbonyl]-th iazolidin-4-ylmethyl}-am ide; 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid {(R)-3-[5-(2-fluoro-phenyl)-2-methyl thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; WO 2008/117241 PCT/IB2008/051110 21 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid {(R)-3-[5-(3-methoxy-phenyl)-2 methyl-th iazole-4-carbonyl]-th iazolidin-4-ylmethyl}-am ide; 6-Methyl-imidazo[2,1-b]thiazole-5-carboxylic acid {(R)-3-[2-methyl-5-(3-trifluoromethyl phenyl)-thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 5 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid {(R)-3-[5-(3,4-difluoro-phenyl)-2 methyl-th iazole-4-carbonyl]-th iazolidin-4-ylmethyl}-am ide; 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid {(R)-3-[5-(2-methoxy-phenyl)-2 methyl-th iazole-4-carbonyl]-th iazolidin-4-ylmethyl}-am ide; 6-Methyl-imidazo[2,1-b]thiazole-5-carboxylic acid {(R)-3-[2-methyl-5-(3-trifluoromethyl 10 phenyl)-oxazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 6-Methyl-imidazo[2,1-b]thiazole-5-carboxylic acid [(R)-3-(2-methyl-5-p-tolyl-oxazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl oxazole-4-carbonyl]-th iazolidin-4-ylmethyl}-amide; 15 6-Methyl-imidazo[2,1-b]thiazole-5-carboxylic acid [(R)-3-(2-methyl-4-p-tolyl-thiazole-5 carbonyl)-thiazolidin-4-ylmethyl]-amide; 6-Methyl-imidazo[2,1-b]thiazole-5-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-oxazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid {(R)-3-[5-(3,4-dichloro-phenyl)-2 20 methyl-th iazole-4-carbonyl]-th iazolidin-4-ylmethyl}-am ide; 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid {(R)-3-[5-(4-methoxy-phenyl)-2 methyl-oxazole-4-carbonyl]-thiazolidin-4-ylmethyl}-am ide; 6-Methyl-imidazo[2,1-b]thiazole-5-carboxylic acid [(R)-3-(4'-methyl-biphenyl-2 carbonyl)-thiazolidin-4-ylmethyl]-amide; 25 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid [(R)-3-(3',4'-dimethyl-biphenyl-2 carbonyl)-thiazolidin-4-ylmethyl]-amide; 6-Methyl-imidazo[2,1-b]thiazole-5-carboxylic acid [(R)-3-(3'-methyl-biphenyl-2 carbonyl)-thiazolidin-4-ylmethyl]-amide; 6-Methyl-imidazo[2,1-b]thiazole-5-carboxylic acid [(R)-3-(3'-methoxy-biphenyl-2 30 carbonyl)-thiazolidin-4-ylmethyl]-amide; 6-Methyl-imidazo[2,1-b]thiazole-5-carboxylic acid [(R)-3-(4'-fluoro-biphenyl-2-carbonyl) thiazolidin-4-ylmethyl]-amide; 6-Methyl-imidazo[2,1-b]thiazole-5-carboxylic acid [(R)-3-(4'-methoxy-biphenyl-2 carbonyl)-thiazolidin-4-ylmethyl]-amide; and WO 2008/117241 PCT/IB2008/051110 22 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid [(R)-3-(biphenyl-2-carbonyl) thiazolidin-4-ylmethyl]-amide. The compounds of formula (1) and their pharmaceutically acceptable salts can be used as medicaments, e.g. in the form of pharmaceutical compositions for enteral or 5 parenteral administration. The production of the pharmaceutical compositions can be effected in a manner which will be familiar to any person skilled in the art (see for example Remington, The Science and Practice of Pharmacy, 21st Edition (2005), Part 5, "Pharmaceutical Manufacturing" [ublished by Lippincott Williams & Wilkins]) by bringing the described 10 compounds of formula (1) or their pharmaceutically acceptable salts, optionally in combination with other therapeutically valuable substances, into a galenical administration form together with suitable, non-toxic, inert, therapeutically compatible solid or liquid carrier materials and, if desired, usual pharmaceutical adjuvants. The present invention also relates to a method for the prevention or treatment of a 15 disease or disorder mentioned herein comprising administering to a subject a pharmaceutically active amount of a compound of formula (1). The compounds according to formula (1) may be used for the preparation of a medicament and are suitable for the prevention or treatment of diseases selected from the group consisting of dysthymic disorders including major depression and 20 cyclothymia, affective neurosis, all types of manic depressive disorders, delirium, psychotic disorders, schizophrenia, catatonic schizophrenia, delusional paranoia, adjustment disorders and all clusters of personality disorders; schizoaffective disorders; anxiety disorders including generalized anxiety, obsessive compulsive disorder, posttraumatic stress disorder, panic attacks, all types of phobic anxiety and avoidance; 25 separation anxiety; all psychoactive substance use, abuse, seeking and reinstatement; all types of psychological or physical addictions, dissociative disorders including multiple personality syndromes and psychogenic amnesias; sexual and reproductive dysfunction; psychosexual dysfunction and addiction; tolerance to narcotics or withdrawal from narcotics; increased anaesthetic risk, anaesthetic responsiveness; 30 hypothalamic-adrenal dysfunctions; disturbed biological and circadian rhythms; sleep disturbances associated with diseases such as neurological disorders including neuropathic pain and restless leg syndrome; sleep apnea; narcolepsy; chronic fatigue syndrome; insomnias related to psychiatric disorders; all types of idiopathic insomnias and parasomnias; sleep-wake schedule disorders including jet-lag; all dementias and WO 2008/117241 PCT/IB2008/051110 23 cognitive dysfunctions in the healthy population and in psychiatric and neurological disorders; mental dysfunctions of aging; all types of amnesia; severe mental retardation; dyskinesias and muscular diseases; muscle spasticity, tremors, movement disorders; spontaneous and medication-induced dyskinesias; neurodegenerative 5 disorders including Huntington's, Creutzfeld-Jacob's, Alzheimer's diseases and Tourette syndrome; Amyotrophic lateral sclerosis; Parkinson's disease; Cushing's syndrome; traumatic lesions; spinal cord trauma; head trauma; perinatal hypoxia; hearing loss; tinnitus; demyelinating diseases; spinal and cranial nerve diseases; ocular damage; retinopathy; epilepsy; seizure disorders; absence seizures, complex 10 partial and generalized seizures; Lennox-Gastaut syndrome; migraine and headache; pain disorders; anaesthesia and analgesia; enhanced or exaggerated sensitivity to pain such as hyperalgesia, causalgia, and allodynia; acute pain; burn pain; atypical facial pain; neuropathic pain; back pain; complex regional pain syndrome I and II; arthritic pain; sports injury pain; dental pain; pain related to infection e.g. by HIV; post 15 chemotherapy pain; post-stroke pain; post-operative pain; neuralgia; osteoarthritis; conditions associated with visceral pain such as irritable bowel syndrome; eating disorders; diabetes; toxic and dysmetabolic disorders including cerebral anoxia, diabetic neuropathies and alcoholism; appetite, taste, eating, or drinking disorders; somatoform disorders including hypochondriasis; vomiting/nausea; emesis; gastric 20 dyskinesia; gastric ulcers; Kallman's syndrome (anosmia); impaired glucose tolerance; intestinal motility dyskinesias; hypothalamic diseases; hypophysis diseases; hyperthermia syndromes, pyrexia, febrile seizures, idiopathic growth deficiency; dwarfism; gigantism; acromegaly; basophil adenoma; prolactinoma; hyperprolactinemia; brain tumors, adenomas; benign prostatic hypertrophy, prostate 25 cancer; endometrial, breast, colon cancer; all types of testicular dysfunctions, fertility control; reproductive hormone abnormalities; hot flashes; hypothalamic hypogonadism, functional or psychogenic amenorrhea; urinary bladder incontinence; asthma; allergies; all types of dermatitis, acne and cysts, sebaceous gland dysfunctions; cardiovascular disorders; heart and lung diseases, acute and congestive heart failure; hypotension; 30 hypertension; dyslipidemias, hyperlipidemias, insulin resistance; urinary retention; osteoporosis; angina pectoris; myocardial infarction; arrhythmias, coronary diseases, left ventricular hypertrophy; ischemic or haemorrhagic stroke; all types of cerebrovascular disorders including subarachnoid haemorrhage, ischemic and hemorrhagic stroke and vascular dementia; chronic renal failure and other renal WO 2008/117241 PCT/IB2008/051110 24 diseases; gout; kidney cancer; urinary incontinence; and other diseases related to general orexin system dysfunctions. Compounds of formula (1) are particularly suitable for use in the treatment of diseases or disorders selected from the group consisting of all types of sleep disorders, of 5 stress-related syndromes, of psychoactive substance use and abuse, of cognitive dysfunctions in the healthy population and in psychiatric and neurologic disorders, of eating or drinking disorders. Eating disorders may be defined as comprising metabolic dysfunction; dysregulated appetite control; compulsive obesities; emeto-bulimia or anorexia nervosa. 10 Pathologically modified food intake may result from disturbed appetite (attraction or aversion for food); altered energy balance (intake vs. expenditure); disturbed perception of food quality (high fat or carbohydrates, high palatability); disturbed food availability (unrestricted diet or deprivation) or disrupted water balance. Drinking disorders include polydipsias in psychiatric disorders and all other types of excessive 15 fluid intake. Sleep disorders include all types of parasomnias, insomnias, narcolepsy and other disorders of excessive sleepiness, sleep-related dystonias; restless leg syndrome; sleep apneas; jet-lag syndrome; shift-work syndrome, delayed or advanced sleep phase syndrome or insomnias related to psychiatric disorders. Insomnias are defined as comprising sleep disorders associated with aging; intermittent treatment of 20 chronic insomnia; situational transient insomnia (new environment, noise) or short-term insomnia due to stress; grief; pain or illness. Insomnia also include stress-related syndromes including post-traumatic stress disorders as well as other types and subtypes of anxiety disorders such as generalized anxiety, obsessive compulsive disorder, panic attacks and all types of phobic anxiety and avoidance; psychoactive 25 substance use, abuse, seeking and reinstatement are defined as all types of psychological or physical addictions and their related tolerance and dependence components. Cognitive dysfunctions include deficits in all types of attention, learning and memory functions occurring transiently or chronically in the normal, healthy, young, adult or aging population, and also occurring transiently or chronically in psychiatric, 30 neurologic, cardiovascular and immune disorders. In a further preferred embodiment of the invention compounds of formula (1) are particularly suitable for use in the treatment of diseases or disorders selected from the group consisting of sleep disorders that comprises all types of insomnias, narcolepsy and other disorders of excessive sleepiness, sleep-related dystonias, restless leg WO 2008/117241 PCT/IB2008/051110 25 syndrome, sleep apneas, jet-lag syndrome, shift-work syndrome, delayed or advanced sleep phase syndrome or insomnias related to psychiatric disorders. In another preferred embodiment of the invention compounds of formula (1) are particularly suitable for use in the treatment of diseases or disorders selected from the 5 group consisting of cognitive dysfunctions that comprise deficits in all types of attention, learning and memory functions occurring transiently or chronically in the normal, healthy, young, adult or aging population, and also occurring transiently or chronically in psychiatric, neurologic, cardiovascular and immune disorders. In another preferred embodiment of the invention compounds of formula (1) are 10 particularly suitable for use in the treatment of diseases or disorders selected from the group consisting of eating disorders that comprise metabolic dysfunction; dysregulated appetite control; compulsive obesities; emeto-bulimia or anorexia nervosa. In another preferred embodiment of the invention compounds of formula (1) are particularly suitable for use in the treatment of diseases or disorders selected from the 15 group consisting of psychoactive substance use and abuse that comprise all types of psychological or physical addictions and their related tolerance and dependence components. Preparation of compounds of formula (1): 20 A further aspect of the invention is a process for the preparation of compounds of formula (1). Compounds according to formula (1) of the present invention can be prepared according to the general sequence of reactions outlined in the schemes below wherein A, D, X, R 1 and R 2 are as defined for formula (1). The compounds obtained may also be converted into pharmaceutically acceptable salts thereof in a 25 manner known per se. In general, all chemical transformations can be performed according to well-known standard methodologies as described in the literature or as described in the procedures below.
    WO 2008/117241 PCT/IB2008/051110 26 <N NH 2 S H
    R
    1 COOH N RR S H S H OT N R1 A-COOH QN f R < 0 0 N N (1) (2) H 0 A:K O Scheme 1: Preparation of compounds of formula (1) Thiazolidine derivatives of formula (1) may be prepared by reacting a thiazolidine derivative of structure (3) with an acid of the general formula A-COOH in a polar aprotic 5 solvent such as DMF, THF, DCM at RT in the presence of a coupling reagent such as TBTU, EDC/HOAt, HATU in the presence of a base such as TEA, DIPEA, DMAP. Acids of the general formula A-COOH are commercially available or synthesized according to methods described below. Thiazolidine derivates of structure (3) may be prepared by treatment of compounds of 10 structure (2) with acids such as HCI in dioxane, TFA in DCM, neat TFA at RT. Compounds of structure (3) may be used as free base or salts thereof such as the hydrochloride salt. A compound of structure (2) may be prepared by reacting 4-aminomethyl-thiazolidine 3-carboxylic acid tert-butyl ester of structure (1), which is commercially available, with 15 an acid of the general formula R 1 -COOH in a polar aprotic solvent such as DMF, THF, DCM at RT in the presence of a coupling reagent such as TBTU, EDC, HATU in presence or absence of additives such as HOBt, HOAt in the presence of a base such as TEA, DIPEA, DMAP. Acids of the general formula R'COOH are commercially available, or synthesized according to methods described below. 20 Preparation of carboxylic acids A-COOH Carboxylic acid derivatives A-COOH wherein A represents a thiazole-4-yl derivative are commercially available or can be synthesised according to scheme 2. S 0 0 D-CHO Cl 0 R 2ANH 2 2 N-I -O' 2 N ICOOH Cl- - D rAO- R R D, (5) (6) (7) (8) Scheme 2: Synthesis of carboxylic acids A-COOH wherein A represents a thiazole-4-yl 25 derivative WO 2008/117241 PCT/IB2008/051110 27 By reaction of methyl dichloroacetate (5) with an aldehyde of the formula D-CHO in the presence of a base such as KOtBu in an aprotic polar solvent such as THF at RT 3-chloro-2-oxo-propionic acid ester derivatives (6) are obtained. Compounds of structure (6) can be transformed by reaction with commercially available thioamides 5 R 2
    -C(S)-NH
    2 at RT in solvents such as MeCN to provide thiazol-4-carboxylic acid ester derivatives (7). Saponification of the ester function using methods known in the art such as treatment with a base such as sodium hydroxide in a solvent such as methanol provides the corresponding thiazol-4-carboxylic acid derivatives (8). Aldehydes of formula D-CHO are commercially available or well known in the art. 10 Carboxylic acid derivatives A-COOH wherein A represents a thiazole-5-yl derivative which are commercially available or synthesised according to scheme 3. Og0 S O O 0 0 CI' Cl D 0 R NH 2 a I KOH HO R2 D" O- I'>-R 2 -I Cl D N D N (9) (10) (11) (12) Scheme 3: Synthesis of carboxylic acids A-COOH wherein A represents a thiazole-5-yl derivative 15 By refluxing a commercially available 3-oxo-propionic acid ester derivative (9) with S0 2 Cl 2 in a solvent such as CHCI 3 the corresponding 2-chloro-3-oxo-propionic acid ester derivatives (10) can be obtained. Compounds of structure (10) can be transformed by reaction with commercially available thioamides R 2
    -C(S)-NH
    2 at reflux temperature in solvents such as THF in presence of a base such as NaHCO 3 to the 20 corresponding thiazol-5-carboxylic acid ester derivatives (11). Saponification of the ester function using methods known in the art such as treatment with a base such as KOH in a solvent such as ethanol provides the corresponding thiazol-5-carboxylic acid derivatives (12). Carboxylic acid derivatives A-COOH wherein A represents a oxazole-4-yl derivative 25 which are commercially available or synthesised according to scheme 4.
    WO 2008/117241 PCT/IB2008/051110 28 0 0 0 0 NaNO 2 0 0 0 0 0 D O~ D 0Z N~~OH HgCI 2 ,Zn H NOH N, (13) (14) 0 (15) 00 0 O CI0 N NaOH N OH 0 D -D (16) (17) Scheme 4: Synthesis of carboxylic acids A-COOH wherein A represents an oxazole-4 yl derivative By reaction of a commercially available 3-oxo-propionic acid ester derivative (13) with 5 an aqueous solution of sodium nitrite in presence of an acid such as glacial acetic acid the corresponding oxime derivative (14) can be obtained. The 2-acetamido-3-oxo propionic acid ester derivative (15) can be synthesized from compounds of structure (14) using acetic anhydride in presence of an acid such as glacial acetic acid and catalytic amounts of metal chlorides such as mercury chloride and zinc powder. 10 Cyclization to the corresponding corresponding oxazole-4 carboxylic acid ester derivative (16) can be achieved under dehydrating conditions such as thionyl chloride in chloroform. Saponification of the ester function using methods known in the art such as treatment with a base such as NaOH in solvent mixtures such as ethanol/water provides the corresponding oxazole-4 carboxylic acid derivative (17). 15 Carboxylic acid derivatives A-COOH wherein A represents a phenyl-2-yl derivative are commercially available or can be synthesised according to scheme 5. 0 H D-Br or D-1 H D-B(OH)2 O 00 BH - OH. 4 2 .0 OH OH Pd(PPh3)4 D Pd(PPh 3
    )
    4 Br, I (18) Na 2
    CO
    3 Na 2 CO3 Scheme 5: Synthesis of carboxylic acids A-COOH wherein A represents a phenyl-2-yl derivative WO 2008/117241 PCT/IB2008/051110 29 Reaction of commercially available (2-carboxyphenyl)-boronic acid derivatives (18) or esters thereof with commercially available aryl-bromides or aryl-iodides of formula D-Br or D-I in presence of a catalyst such as Pd(PPh 3
    )
    4 and a base such as Na 2
    CO
    3 under heating in a solvent such as toluene, dioxane, THF provides, after saponification, if 5 needed, of the ester using well known methods, the corresponding phenyl 2-carboxylic acid derivatives (19). Alternatively, reaction of commercially available 2-bromo-, or 2-iodo-benzoic acid, or esters thereof, with commercially available boronic acid derivatives of formula D-B(OH) 2 using the conditions described before provides the corresponding phenyl-2-carboxylic acid derivatives (19). 10 Synthesis of Carboxylic Acids R'-COOH Carboxylic acids of formula R 1 -COOH are commercially available or well known in the art (Lit. e.g. W02001/96302; T. Eicher, S. Hauptmann "The chemistry of Heterocycles: Structure, Reactions, Syntheses, and Applications", 2nd Edition 2003, Wiley, ISBN 978-3-527-30720-3). 15 Carboxylic acid derivatives R 1 -COOH which represent an imidazo[2,1-b]thiazole 2-carboxylic acid derivative are commercially available or can be synthesised according to scheme 6. Pathway A: By reaction of 2-chloro-3-oxo-butyric acid methyl ester (20) with thiourea the amino-thiazole (21) can be obtained. Transformation to ester (22) can be 20 accomplished with bromoacetaldehydewhich can be generated in-situ from bromoacetaldehyde diethylacetal under acidic conditions. After saponification with bases such as sodium hydroxide the desired acid (23) can be obtained. Pathway A S Br'^YO- O H 2 N NH 2 -- NH 2 O N NaOH Cl 0 S HCI O (20) (21) (22) (23) WO 2008/117241 PCT/IB2008/051110 30 Pathway B O 0 RaN O Br fO" R Br Rb>S NN 0 N~ RbI NH2 Rb) N Rb CI > N, R / Rb S ' (24) (25) (26) o H02C DBU Ra N NaOH Ra N Rb S N Rb ISN (27) (28) Scheme 6: Synthesis of carboxylic acids R 1 -COOH which represent an imidazo [2,1 -b]thiazole-2-carboxylic acid derivative 5 Pathway B: By heating a compound of structure (24) with NN-dimethylformamide dimethylacetal in a solvent such as toluene formamidine derivatives (25) can be obtained. They can be alkylated with ethyl bromoacetate yielding the respective thiazolium bromide (26) which can be cyclised with strong bases such as DBU to the ester (27). Saponification of the ester function using methods known in the art such as 10 treatment with a base such as NaOH in a solvent such as ethanol/water provides the corresponding imidazo[2,1 -b]thiazole-2-carboxylic acid derivatives (28). Carboxylic acid derivatives R 1 -COOH which represent a pyrrolo[2,1-b]thiazole 7-carboxylic acid derivative can be synthesised according to scheme 7 By reaction of 2-methylsulfanylthiazole (29) with trimethylsilylmethyl 15 trifluoromethanesulfonate followed by cyclisation of the resulting thiazolinium salt by reaction with ethyl propiolate in the presence of caesium fluoride, the pyrrolo[2,1 b]thiazole (30) can be obtained. Saponification of the ester function using methods known in the art such as treatment with a base such as NaOH in a solvent such as EtOH/ water provides the corresponding pyrrolo[2,1-b]thiazole-7-carboxylic acid 20 derivative (31) (Berry C.R. et al., Organic Letters, 2007, 9, 21, 4099-4102).
    WO 2008/117241 PCT/IB2008/051110 31 TMS N TfO'^'TMS TN Tfo 0-C02Et NNaOH N S S CO2Et C0 2 H CsF S (29) (30) (31) NBS Br [ CO2Et
    (CH
    3
    )
    2 Zn/ Pd(dppf)C1 2 N ENaOH N(CO2 s C0 2 Et C 0 2 H (32) (33) Scheme 7: Synthesis of carboxylic acids R 1 -COOH which represent a pyrrolo [2,1 -b]thiazole-7-carboxylic acid derivative Bromination of (30) by reaction with NBS followed by methylation of the resulting crude 5 ethyl 6-bromo-pyrrolo[2,1-b]thiazole-7-carboxylate by reaction with dimethylzinc in the presence of a palladium catalyst such as Pd(dppf)C1 2 gave the ester (32). Saponification of the ester function using methods known in the art such as treatment with a base such as NaOH in a solvent such as EtOH/ water provides the corresponding 6-methyl-pyrrolo[2,1 -b]thiazole-7-carboxylic acid derivative (33). 10 Carboxylic acid derivatives R 1 -COOH which represent a 3,4-dihydro-2H benzo[1,4]oxazinyl- or 3-oxo-3,4-dihydro-2H-benzo[1,4]oxazinyl-carboxylic acid derivative can be synthesised according to the literature according to schemes 8 and 9. Esterification of 3-hydroxy-anthranilic acid (34) with concentrated sulphuric acid in EtOH provides the corresponding ethyl ester (35). Cyclisation with acetyl chloride in 15 presence of a base such as K 2
    CO
    3 in a solvent such as DMF provides 3-oxo-3,4 dihydro-2H-benzo[1,4]oxazine derivatives (36). Compounds of structure (36) can optionally be alkylated with alkylating reagents such as methyl iodide in presence of a base such as K 2
    CO
    3 . Saponification with a base such as NaOH in a solvent such as EtOH/ water leads to the corresponding acids (37) or (38). Reduction of compounds of 20 structure (36) with NaBH 4 in the presence of BF 3 -diethyl etherate leads to the corresponding 3,4-dihydro-2H-benzo[1,4]oxazine derivative which can optionally be WO 2008/117241 PCT/IB2008/051110 32 alkylated and/or saponified as described before to provide the corresponding acids (40) or (41) (Kuroita T. et al, Chemical Pharmaceutical Bulletin 1996,44,4,756-764). HO O H | N O K K 0 COOH O 0 0H (37)
    NH
    2
    NH
    2 CI K a NyO OH ~ OH 0 HOO0 (34) (35) (36) HO 0 NaBH 4 / BF 3 .0(C 2
    H
    5
    )
    2 (38) r HO H 0 0 H HO O N N N 0 0 0 (40) (39) (41) 5 Scheme 8: Synthesis of carboxylic acids R 1 -COOH which represent a 3,4-dihydro-2H benzo[1,4]oxazinyl- or 3-oxo-3,4-dihydro-2H-benzo[1,4]oxazinyl-carboxylic acid derivative 0OH O &N 000 0 0 H OH OH CI NO (45
    NO
    2
    NH
    2 O O OH H0 (42) (43) (44) N (46) Scheme 9: Synthesis of carboxylic acids R 1 -COOH which represent a 10 3,4-dihydro-2H-benzo[1,4] oxazinyl-carboxylic acid derivative WO 2008/117241 PCT/IB2008/051110 33 Hydrogenation of methyl 3-nitrosalicylate (42) in presence of a palladium catalyst provides the aniline derivative (43) which can be cyclized with chloroacetyl chloride as described before to the ester (44). Reduction of compounds of structure (44) with NaBH 4 in the presence of BF 3 -diethyl etherate leads to the corresponding 3,4-dihydro 5 2H-benzo[1,4]oxazine derivative which can optionally be alkylated and/or saponified as described before to provide the corresponding acids (45) or (46) (Kuroita T. et al, Chemical Pharmaceutical Bulletin 1996, 44, 4, 756-764). Carboxylic acid derivatives R 1 -COOH which represent a benzooxazole-4-carboxylic acid derivative can be synthesised according to the literature according to schemes 10 10 and 11. 00 00 OH
    NH
    2 Cl l N NaOH N OH PPTS/TEA 0 & O (47) (48) (49) Scheme 10: Synthesis of carboxylic acids R 1 -COOH which represent a benzooxazole-4-carboxylic acid derivative By cyclisation of ethyl 2-amino-3-hydroxybenzoate (47) with acetyl chloride in the 15 presence of PPTS and TEA, the ester (48) can be obtained (Goldstein S.W. et al, Journal of Heterocyclic Chemistry, 1990, 27, 335-336). Saponification of the ester function using methods known in the art such as treatment with a base such as NaOH in a solvent such as EtOH / water provides the corresponding 2-methyl-benzooxazole 4-carboxylic acid derivative (49). 0OH OEt O OH OEt 0OH -(-OEt H-(-OEt O OEt OH OEt O N PTSA NH 2 PTSA N 20 (52) (50) (51) Scheme 11: Synthesis of carboxylic acids R 1 -COOH which represent a benzooxazole 7-carboxylic acid derivative By cyclisation of 3-aminosalicylic acid (50) with triethyl orthoformate in the presence of PTSA, the benzooxazole-7-carboxylic acid (51) can be obtained (W02006/069155). By 25 cyclisation of 3-aminosalicylic acid (50) with triethyl orthoacetate in the presence of WO 2008/117241 PCT/IB2008/051110 34 PTSA, the 2-methyl-benzooxazole-7-carboxylic acid (52) can be obtained (W02006/069155) Carboxylic acid derivatives R 1 -COOH which represent a benzothiazole-7-carboxylic acid derivative can be synthesised according to the literature according to schemel2. OOs O OOs ONOOON KSCN/ H 2
    SO
    4 Br 2 S S AcOH ' NH2 ' I I
    NH
    2 18-C-6 NH N N (53) H 2 N S (55) (56) (54) O OH NaOH S N 5 (57) Scheme 12: Synthesis of carboxylic acids R 1 -COOH which represent a benzothiazole 7-carboxylic acid derivative By reaction of methyl 3-aminobenzoate (53) with potassium thiocyanate in the presence of sulfuric acid and crown-ether 18-C-6, the thiourea (54) can be obtained. 10 Cyclisation by reaction with bromine in acetic acid provides the 2-amino-benzothiazole derivative (55). Cleavage of the amino group by reaction with isoamyl nitrite furnishes the ester (56) (W02005/092890). Saponification of the ester function using methods known in the art such as treatment with a base such as NaOH in a solvent such as MeOH/ water provides the corresponding benzothiazole-7-carboxylic acid derivative 15 (57). Carboxylic acid derivatives R 1 -COOH which represent a benzofuran-4-carboxylic acid derivative can be synthesised according to the literature according to schemes 13 and 14. By reaction of methyl 3-hydroxybenzoate (58) with 3-chloro-2-butanone, the ester (59) 20 can be obtained. Cyclisation with sulfuric acid provides the 2,3-dimethyl-benzofurane derivative (60) (Kawase Y. et al, Bulletin of the Chemical Sociaty of Japan, 1967, 40, 5, 1224-1231). Saponification of the ester function using methods known in the art such as treatment with a base such as NaOH in a solvent such as MeOH/ water provides the corresponding 2,3-dimethyl-benzofuran-4-carboxylic acid derivative (61). On the other WO 2008/117241 PCT/IB2008/051110 35 hand, reaction of methyl 3-hydroxybenzoate (58) with crotyl bromide furnishes the ester (62) which after reaction in N,N-dimethylaniline provides the ester (63). Ozonolysis followed by reaction with PTSA gives the 3-methyl-benzofurane derivative (64) (Mohamadi F. et al, Journal of Medicinal Chemistry, 1994, 37, 232-239 and EP58906). 5 Saponification of the ester function using methods known in the art such as treatment with a base such as NaOH in a solvent such as MeOH/ water provides the corresponding 3-methylbenzofuran-4-carboxylic acid derivative (65). CI O OsO Os O Os O OH o O H 2
    SO
    4 NaOH OH K 2
    CO
    3 / KI 0 0O (58) (59) (60) (61) sf Br K2CO3/ KI KII O 0,~ 0 0 1)03 0 0,.. 0 OH PhN(Me) 2 2) PTSA " NaOH &0 OH 6 O (62) (63) (64) (65) Scheme 13: Synthesis of carboxylic acids R 1 -COOH which represent a 2,3-dimethyl 10 benzofuran-4-carboxylic acid derivative O H OH 0OOH Pd(MeCN) 2 Cl 2 NaCIO 2 ~OH 0 on (66) (67) (68) Scheme 14: Synthesis of carboxylic acids R 1 -COOH which represent a 2-methylbenzofuran-4-carboxylic acid derivative By cyclisation of 2-allyl-3-hydroxybenzaldehyde (66) with a palladium catalyst such as 15 bis(acetonitrile)dichloropalladium in the presence of 1,4-benzoquinone and lithium chloride, the 2-methyl-benzofurane carbaldehyde (67) can be obtained (Danheiser R.L. et al, Organic Letters, 2005, 7, 18, 3905-3908). Oxidation of the aldehyde function with sodium chlorite in the presence of a scavenger such as 2-methyl-2-butene furnishes the corresponding 2-methylbenzofuran-4-carboxylic acid (68).
    WO 2008/117241 PCT/IB2008/051110 36 Carboxylic acid derivatives R 1 -COOH which represent a benzofuran-4-carboxylic acid derivative and R represent Cl, F or CF 3 can be synthesised according to the literature according to scheme 15. 0OOH OO'-- OO'- 0OH R NaOH R /NaOH R 0 R/' / - 0 R 0 (77) (76) (74) (75) Pd(MeCN) 2 Cl 2 O OH 1) H 2
    SO
    4 / EtOH 2) Br 0 O,_0 0 1)0 Os
    K
    2
    CO
    3 / KI PhN(Me) 2 2) PTSA -& OH ' 0 R OH O (69) (70) (71) (72) O OH NaOH -/ &I (73) 5 Scheme 15: Synthesis of carboxylic acids R 1 -COOH which represent a substituted benzofuran-4-carboxylic acid derivative By esterification of phenol derivative (69) with EtOH in the presence of an acid such as sulfuric acid followed by alkylation by reaction with allyl bromide in the presence of a
    K
    2
    CO
    3 and KI, the alkyl-ether derivative (70) can be obtained. Claisen rearrangement 10 by reaction with N,N-dimethylaniline furnishes the phenol derivative (71). Ozonolysis followed by reaction with PTSA provides the benzofurane derivative (72). On the other hand ozonolysis of (71) in the presence of MeOH furnishes the dihydro-benzofurane derivative (74). Saponification of the ester function of (72) and (74) using methods known in the art such as treatment with a base such as NaOH in a solvent such as 15 EtOH/ water provide the corresponding benzofuran-4-carboxylic acid derivatives (73) and (75). Furthermore, cyclisation of (71) with a palladium catalyst such as bis(acetonitrile)dichloropalladium in the presence of 1,4-benzoquinone and lithium chloride, the 2-methylbenzofurane derivative (76) can be obtained (Danheiser R.L. et al, Organic Letters, 2005, 7, 18, 3905-3908). Saponification of the ester function of (76) WO 2008/117241 PCT/IB2008/051110 37 using methods known in the art such as treatment with a base such as NaOH in a solvent such as EtOH/ water provide the corresponding 2-methyl-benzofuran-4 carboxylic acid derivatives (77). Carboxylic acid derivatives R 1 -COOH which represent a benzofuran-4-carboxylic acid 5 derivative can be synthesised according to the literature according to scheme 16. O 0 , 1) , B r
    K
    2
    CO
    3 / KI O OO 2) PhN(Me) 2 - mCPBA OH ~OH I 1 OH NaHCO 3 On0 (58) (78) (79) 1) AC 2 0 0 0, 1) K 2 00 3 0O 2)DQ 2)NaOH OH (80) (81) Scheme 16: Synthesis of carboxylic acids R-COOH which represent a 2 hydroxymethylbenzofuran-4-carboxylic acid derivative By alkylation of methyl 2-hydroxybenzoate (58) with allyl bromide in the presence of 10 K 2
    CO
    3 and KI followed by Claisen rearrangement by reaction with N,N-dimethylaniline the phenol derivative (78) can be obtained. Cyclisation by reaction with mCPBA in presence of a base such as NaHCO 3 furnishes the desired dihydro-benzofurane derivative (79). Acetylation by reaction with acetic anhydride followed by oxidation with DDQ provides the corresponding benzofurane derivative (80). Cleavage of the acetyl 15 group by reaction with K 2
    CO
    3 followed by saponification of the ester function using methods known in the art such as treatment with a base such as NaOH in a solvent such as MeOH/ water provide the corresponding 2-hydroxymethylbenzofuran-4 carboxylic acid derivatives (81). Carboxylic acid derivatives R 1 -COOH which represent a 2-fluorobenzofuran-4 20 carboxylic acid derivative can be synthesised according to the literature according to scheme 17. Specific electrophilic fluorination of benzofuran-4-carboxylic acid (82) (Eissenstat M.A. et al, Journal of Medicinal Chemistry 1995, 38, 16, 3094-3105) by reaction with tert butyl lithium followed by reaction with NFSI (Torrado A. et al Bioorganic Medicinal WO 2008/117241 PCT/IB2008/051110 38 Chemistry 2004, 12, 5277-5295 and Differling E. et al Synlett, 1991, 1, 187-189) provides the desired 2-fluorobenzofuran-4-carboxylic acid (83). O OH O OH 1) t-BuLi 2) NFSI |F 0 ~0 (82) (83) Scheme 17: Synthesis of carboxylic acids R 1 -COOH which represent a 2 5 fluorobenzofuran-4-carboxylic acid derivative Compounds which contain a 2-trifluoromethylbenzofurane moiety can be synthesised according to the literature according to scheme 18. 5 NH2 Boc (85) 1) CF 3
    CO
    2 Et 2) TFA 3) A-C0 2 H/ TBTU/ DIPEA 4) sat. K 2 CO3 S NH20 O OH 0 OH N (86) HN K ~Bu i" A O N 0 0 TBTU/ DIPEA A O (82) (84) (87)
    FSO
    2
    CF
    2
    CO
    2 Me S- HN
    CF
    3 Cul/ HMPA/ DMF N A (88) Scheme 18: Synthesis of compounds containing a 2-trifluoromethyl-benzofurane 10 moiety Specific electrophilic iodination of benzofuran-4-carboxylic acid (82) by reaction with tert-butyl lithium followed by reaction with iodine provides the desired 2-iodo benzofuran-4-carboxylic acid (84). Amide coupling using classical methodology (i.e.
    WO 2008/117241 PCT/IB2008/051110 39 TBTU/ DIPEA) with n-acyl-thiazolidine (86) derivative furnishes bis-N-acyl-thiazolidine intermediate (87). The N-acyl-thiazolidine derivatives (86) can be prepared by trifluoroacetylation of commercially available (R)-4-Aminomethyl-thiazolidine-3 carboxylic acid tert-butyl ester (85) with ethyl trifluoroacetate followed by removal of the 5 Boc-protecting group with TFA, acylation with A-CO 2 H using classical amide coupling methodology (TBTU/ DIPEA) and finally removal of the trifluoroacetyl-protecting group by reaction with sat. K 2
    CO
    3 . Trifluoromethylation of (87) with methyl (fluorosulfonyl)difluoroacetate in the presence of copper (1) iodide in a mixture of HMPA/ DMF (Chen Q. et al Journal of Chemical Society: Chemical Communications 10 1989, 11, 705-706 and Chen Q. et al Journal of Fluorine Chemistry. 1991, 55, 3, 291 298) provides the 2-trifluoromethyl-benzofurane thiazolidine derivatives (88). Whenever the compounds of formula (1) are obtained in the form of mixtures of enantiomers, the enantiomers can be separated using methods known to one skilled in the art: e.g. by formation and separation of diastereomeric salts or by HPLC over a 15 chiral stationary phase such as a Regis Whelk-01(R,R) (10 jim) column, a Daicel ChiralCel OD-H (5-10 jim) column, or a Daicel ChiralPak IA (10 jim) or AD-H (5 jim) column. Typical conditions of chiral HPLC are an isocratic mixture of eluent A (ethanol, in presence or absence of an amine such as TEA, diethylamine) and eluent B (hexane), at a flow rate of 0.8 to 150 mL/min. 20 Experimental Section Abbrevations (as used herein and in the description above): aq. aqueous Boc tert-Butoxycarbonyl BSA Bovine serum albumine 25 CHO Chinese hamster ovary conc. Concentrated d Day(s) DBU 1,8-Diazabicyclo[5.4.0]undec-7-ene DCM Dichloromethane 30 DDQ 2,3-Dichloro-5,6-dicyano-1,4-benzoquinone DIPEA Diisopropylethylamine DMAP 4-Dimethylaminopyridine DMF NN-Dimethylformamide WO 2008/117241 PCT/IB2008/051110 40 dppf diphenylphosphinoferrocene EDC 1 -Ethyl-3-(3-dimethylaminopropyl)-carbodiimide eq Equivalent(s) ES Electron spray 5 Et Ethyl ether diethylether EtOAc Ethyl acetate FCS Foatal calf serum FLIPR Fluorescent imaging plate reader 10 h Hour(s) HATU (O-(7-azabenzotriazol-1-yl)-1,1,3,3-tetramethyl-uronium hexafluorphoshate HBSS Hank's balanced salt solution HCI Hydrochloric acid 15 HEPES 4-(2-hydroxyethyl)-piperazine-1 -ethanesulfonic acid HMPA Hexamethylphosphoramide HOAt 1 -hydroxy-7-azabenzotriazole HOBt 1 -hydroxy-benzotriazole HPLC High performance liquid chromatography 20 KOtBu Potassium tert. butoxide LC Liquid chromatography M Molar(ity) Me Methyl MeCN Acetonitrile 25 mCPBA meta-chloroperoxybenzoic acid MeOH Methanol min Minute(s) MS Mass spectroscopy NBS N-bromosuccinimide 30 NFSI N-Fluorobenzenesulfonimide prep. Preparative PPTS Pyridinium 4-toluenesulfonate PTSA p-Toluenesulfonic acid RT Room temperature 35 sat Saturated WO 2008/117241 PCT/IB2008/051110 41 tR Retention time TBTU O-(Benzotriazol-1-yl)-N,N,N',N'-tetramethyluronium tetrafluoroborate TEA Triethylamine TFA trifluoroacetic acid 5 THF Tetrahydrofuran I-Chemistry All temperatures are stated in 0C. Compounds are characterized by 'H-NMR (300 MHz: Varian Oxford or 400 MHz: Bruker Avance); chemical shifts are given in ppm relative to the solvent used; multiplicities: s = singlet, d = doublet, t = triplet; p = pentuplet, hex = 10 hexet, hept = heptet, m = multiplet, br = broad, coupling constants are given in Hz); by LC-MS (Finnigan Navigator with HP 1100 Binary Pump and DAD, column: 4.6x50 mm, Zorbax SB-AQ, 5 jim, 120 A, using two conditions: basic: eluent A: MeCN, eluent B: conc. NH 3 in water (1.0 mL/L), 5% to 95% CH 3 CN; acidic: eluent A: MeCN, eluent B: TFA in water (0.4 mL/L), 5% to 95% CH 3 CN), tR is 15 given in min; by TLC (TLC-plates from Merck, Silica gel 60 F 2 54 ); or by melting point. Compounds are purified by column chromatography on silica gel or by preparative HPLC (column: X-terra RP18, 50x19 mm, 5 jim, gradient: 10-95% MeCN in water containing 0.5 % of formic acid). The following examples illustrate the preparation of pharmacologically active 20 compounds of the invention but do not at all limit the scope thereof. Preparation of precursors and intermediates: A.1 Synthesis of thiazole-carboxylic acid derivatives A.1.1 Synthesis of 3-chloro-2-oxo-propionic ester derivatives (general procedure) 0 D-CHO CI 0 CI 0 D 25 CI 0 A solution of the respective benzaldehyde derivative D-CHO (338 mmol, 1.0 eq) and methyl dichloroacetate (338 mmol, 1.0 eq) in THF (100 mL) is added dropwise to a cold (-600C) suspension of KOtBu (335 mmol, 1.0 eq) in THF (420 mL). After 4 h the mixture is allowed to reach RT, stirred over night and concentrated in vacuo. DCM and 30 ice-cold water are added, the layers are separated and the aqueous layer is extracted WO 2008/117241 PCT/IB2008/051110 42 twice with DCM. The combined organic layers are washed with ice-cold water and brine, dried over MgSO 4 and concentrated in vacuo to give the corresponding 3-chloro 2-oxo-propionic acid methyl ester derivative which is used without further purification. 3-Chloro-2-oxo-3-phenyl-propionic acid methyl ester 5 prepared by reaction of benzaldehyde with methyl dichloroacetate. 3-Chloro-2-oxo-3-m-tolyl-propionic acid methyl ester prepared by reaction of 3-methyl-benzaldehyde with methyl dichloroacetate. 3-Chloro-2-oxo-3-p-tolyl-propionic acid methyl ester prepared by reaction of 4-methyl-benzaldehyde with methyl dichloroacetate. 10 3-Chloro-3-(3-fluoro-phenyl)-2-oxo-propionic acid methyl ester prepared by reaction of 3-fluoro-benzaldehyde with methyl dichloroacetate. 3-Chloro-3-(3,4-dichloro-phenyl)-2-oxo-propionic acid methyl ester prepared by reaction of 3,4-dichloro-benzaldehyde with methyl dichloroacetate. 3-Chloro-3-(3,4-difluoro-phenyl)-2-oxo-propionic acid methyl ester 15 prepared by reaction of 3,4-difluoro-benzaldehyde with methyl dichloroacetate. 3-Chloro-3-(3,4-dimethyl-phenyl)-2-oxo-propionic acid methyl ester prepared by reaction of 3,4-dimethyl-benzaldehyde with methyl dichloroacetate. 3-Chloro-3-(2-fluoro-phenyl)-2-oxo-propionic acid methyl ester prepared by reaction of 2-fluoro-benzaldehyde with methyl dichloroacetate. 20 3-Chloro-3-(4-methoxy-phenyl)-2-oxo-propionic acid methyl ester prepared by reaction of 4-methoxy-benzaldehyde with methyl dichloroacetate. 3-Chloro-3-(3-methoxy-phenyl)-2-oxo-propionic acid methyl ester prepared by reaction of 3-methoxy-benzaldehyde with methyl dichloroacetate. 3-Chloro-3-(2-methoxy-phenyl)-2-oxo-propionic acid methyl ester 25 prepared by reaction of 2-methoxy-benzaldehyde with methyl dichloroacetate. 3-Chloro-3-(4-fluoro-phenyl)-2-oxo-propionic acid methyl ester prepared by reaction of 4-fluoro-benzaldehyde with methyl dichloroacetate. 3-Chloro-2-oxo-3-(3-trifluoromethyl-phenyl)-propionic acid methyl ester prepared by reaction of 3-trifluoromethyl-benzaldehyde with methyl dichloro-acetate. 30 3-Chloro-2-oxo-3-(4-trifluoromethyl-phenyl)-propionic acid methyl ester prepared by reaction of 4-trifluoromethyl-benzaldehyde with methyl dichloroacetate.
    WO 2008/117241 PCT/IB2008/051110 43 A.1.2 Synthesis of 2-methyl-thiazole-4-carboxylic acid methyl ester derivatives (general procedure) S 0 C10
    NH
    2 N 0O, D O S D 0 A solution of thioacetamide (132 mmol, 1.0 eq) in MeCN (250 mL) is added to a 5 mixture of the respective 3-chloro-2-oxo-propionic acid methyl ester derivative (132 mmol, 1.0 eq) and molecular sieves (4A, 12 g) in MeCN (60 mL). After stirring for 5 h the mixture is cooled in an ice-bath and the obtained precipitate is filtered off. The residue is washed with cold MeCN, dried, dissolved in MeOH (280 mL) and stirred at 500C for 6 h. The solvents are removed in vacuo to give the corresponding 2-methyl 10 thiazole-4-carboxylic acid methyl ester derivatives. 2-Methyl-5-m-tolyl-thiazole-4-carboxylic acid methyl ester prepared by reaction of 3-chloro-2-oxo-3-m-tolyl-propionic acid methyl ester with thioacetamide. LC-MS: tR = 0.94 min; [M+H]* = 248.0. 2-Methyl-5-p-tolyl-thiazole-4-carboxylic acid methyl ester 15 prepared by reaction of 3-chloro-2-oxo-3-p-tolyl-propionic acid methyl ester with thioacetamide. LC-MS: tR = 0.93 min; [M+H]* = 248.02. 5-(3-Fluoro-phenyl)-2-methyl-thiazole-4-carboxylic acid methyl ester prepared by reaction of 3-chloro-3-(3-fluoro-phenyl)-2-oxo-propionic acid methyl ester with thioacetamide. LC-MS: tR = 0-91 min; [M+H]* = 252.1. 20 5-(4-Fluoro-phenyl)-2-methyl-thiazole-4-carboxylic acid methyl ester prepared by reaction of 3-chloro-3-(4-fluoro-phenyl)-2-oxo-propionic acid methyl ester with thioacetamide. 'H-NMR (CDC13): 6 = 2.75 (s, 3H); 3.84 (s, 3H); 7.10 (m, 2H); 7.47 (m, 2H). 5-(2-Fluoro-phenyl)-2-methyl-thiazole-4-carboxylic acid methyl ester 25 prepared by reaction of 3-chloro-3-(2-fluoro-phenyl)-2-oxo-propionic acid methyl ester with thioacetamide. LC-MS: tR = 0-90 min; [M+H]* = 251.99. 2-Methyl-5-(3-trifluoromethyl-phenyl)-thiazole-4-carboxylic acid methyl ester prepared by reaction of 3-chloro-3-(3-trifluoromethyl-phenyl)-2-oxo-propionic acid methyl ester with thioacetamide. LC-MS: tR = 0.99 min; [M+H]* = 301.99.
    WO 2008/117241 PCT/IB2008/051110 44 2-Methyl-5-(4-trifluoromethyl-phenyl)-thiazole-4-carboxylic acid methyl ester prepared by reaction of 3-chloro-3-(4-trifluoromethyl-phenyl)-2-oxo-propionic acid methyl ester with thioacetamide. LC-MS: tR = 0.99 min; [M+H]* = 301.99 2-Methyl-5-(3,4-dimethyl-phenyl)-thiazole-4-carboxylic acid methyl ester 5 prepared by reaction of 3-chloro-3-(3,4-dimethyl-phenyl)-2-oxo-propionic acid methyl ester with thioacetamide. LC-MS: tR = 0.96 min; [M+H]* = 262.34. 2-Methyl-5-(3,4-dichloro-phenyl)-thiazole-4-carboxylic acid methyl ester prepared by reaction of 3-chloro-3-(3,4-dichloro-phenyl)-2-oxo-propionic acid methyl ester with thioacetamide. LC-MS: tR = 0-99 min; [M+H]* = 302.22. 10 2-Methyl-5-(3,4-difluoro-phenyl)-thiazole-4-carboxylic acid methyl ester prepared by reaction of 3-chloro-3-(3,4-difluoro-phenyl)-2-oxo-propionic acid methyl ester with thioacetamide. LC-MS: tR = 0.92 min; [M+H]* = 270.29. 5-(4-Methoxy-phenyl)-2-methyl-thiazole-4-carboxylic acid methyl ester prepared by reaction of 3-chloro-3-(4-methoxy-phenyl)-2-oxo-propionic acid methyl 15 ester with thioacetamide. LC-MS: tR = 0-90 min; [M+H]* = 263.93. 5-(3-Methoxy-phenyl)-2-methyl-thiazole-4-carboxylic acid methyl ester prepared by reaction of 3-chloro-3-(3-methoxy-phenyl)-2-oxo-propionic acid methyl ester with thioacetamide. LC-MS: tR = 0-90 min; [M+H]* = 263.87. 5-(2-Methoxy-phenyl)-2-methyl-thiazole-4-carboxylic acid methyl ester 20 prepared by reaction of 3-chloro-3-(2-methoxy-phenyl)-2-oxo-propionic acid methyl ester with thioacetamide. LC-MS: tR = 0.88 min; [M+H]* = 264.05. 5-Phenyl-2-methyl-thiazole-4-carboxylic acid methyl ester prepared by reaction of 3-chloro-3-phenyl-2-oxo-propionic acid methyl ester with thioacetamide. LC-MS: tR = 0.88 min; [M+H]* = 234.23. 25 A.1.3 Synthesis of thiazole-4-carboxylic acid derivatives (general procedure) 0 N O NaOH N COOH S D S D A solution of the respective thiazole-4-carboxylic acid methyl ester (96.2 mmol) in a mixture of THF (150 mL) and MeOH (50 mL) is treated with 1M aq. NaOH (192 mL). After stirring for 3 h a white suspension is formed and the organic volatiles are removed 30 in vacuo. The remaining mixture is diluted with water (100 mL), cooled in an ice-bath WO 2008/117241 PCT/IB2008/051110 45 and acidified (pH = 3-4) by addition of 1M aq. HCI. The suspension is filtered and the residue is washed with cold water. After drying the corresponding 2-methyl-thiazole-4 carboxylic acid derivative is obtained. 2-Methyl-5-m-tolyI-thiazole-4-carboxylic acid 5 prepared by saponification of 2-methyl-5-m-tolyl-thiazole-4-carboxylic acid methyl ester. LC-MS: tR = 0.83 min; [M+H]* = 233.99. 2-Methyl-5-p-tolyI-thiazole-4-carboxylic acid prepared by saponification of 2-methyl-5-p-tolyl-thiazole-4-carboxylic acid methyl ester. LC-MS: tR = 0.83 min; [M+H]* = 234.0. 10 5-(3-Fluoro-phenyl)-2-methyl-thiazole-4-carboxylic acid prepared by saponification of 5-(3-fluoro-phenyl)-2-methyl-thiazole-4-carboxylic acid methyl ester. LC-MS: tR = 0.82 min; [M+H]* = 238.1. 5-(4-Fluoro-phenyl)-2-methyl-thiazole-4-carboxylic acid prepared by saponification of 5-(4-fluoro-phenyl)-2-methyl-thiazole-4-carboxylic acid 15 methyl ester. 1 H-NMR (DMSO-d 6 ): 6 = 2.67 (s, 3H); 7.27 (m, 2H); 7.53 (m, 2H); 12.89 (br.s, 1H). 2-Methyl-5-(3-trifluoromethyl-phenyl)-thiazole-4-carboxylic acid prepared by saponification of 2-methyl-5-(3-trifluoromethyl-phenyl)-thiazole 4-carboxylic acid methyl ester. LC-MS: tR = 0.88 min; [M+H]* = 287.99. 20 2-Methyl-5-(4-trifluoromethyl-phenyl)-thiazole-4-carboxylic acid prepared by saponification of 2-methyl-5-(4-trifluoromethyl-phenyl)-thiazole 4-carboxylic acid methyl ester. LC-MS: tR = 0.90 min; [M+H]* = 287.99. 2-Methyl-5-(3,4-dimethyl-phenyl)-thiazole-4-carboxylic acid prepared by saponification of 2-methyl-5-(3,4-dimethyl-phenyl)-thiazole-4-carboxylic 25 acid methyl ester. LC-MS: tR = 0.97 min; [M+H]* = 382.38. 2-Methyl-5-(3,4-dichloro-phenyl)-thiazole-4-carboxylic acid prepared by saponification of 2-methyl-5-(3,4-dichloro-phenyl)-thiazole-4-carboxylic acid methyl ester. LC-MS: tR = 0.88 min; [M+H]* = 288.22. 2-Methyl-5-(3,4-difluoro-phenyl)-thiazole-4-carboxylic acid 30 prepared by saponification of 2-methyl-5-(3,4-difluoro-phenyl)-thiazole-4-carboxylic acid methyl ester. LC-MS: tR = 0.82 min; [M+H]* = 256.25. 2-Methyl-5-(2-methoxy-phenyl)-thiazole-4-carboxylic acid WO 2008/117241 PCT/IB2008/051110 46 prepared by saponification of 2-methyl-5-(2-methoxy-phenyl)-thiazole-4-carboxylic acid methyl ester. LC-MS: tR = 0.78 min; [M+H]* = 249.98. 2-Methyl-5-(3-methoxy-phenyl)-thiazole-4-carboxylic acid prepared by saponification of 2-methyl-5-(3-methoxy-phenyl)-thiazole-4-carboxylic acid 5 methyl ester. LC-MS: tR = 0.80 min; [M+H]* = 250.04. 2-Methyl-5-(4-methoxy-phenyl)-thiazole-4-carboxylic acid prepared by saponification of 2-methyl-5-(4-methoxy-phenyl)-thiazole-4-carboxylic acid methyl ester. LC-MS: tR = 0.80 min; [M+H]* = 250.04. 2-Methyl-5-phenyl-thiazole-4-carboxylic acid 10 prepared by saponification of 2-methyl-5-phenyl-thiazole-4-carboxylic acid methyl ester. LC-MS: tR = 0.78 min; [M+H]* = 220.01. A.1.4 Synthesis of 2-methyl-4-p-tolyl-thiazole-5-carboxylic acid A mixture of 4-methylbenzoyl acetate (5.52 mmol), sulfuryl chloride (5.52 mmol) in chloroform (3.3 ml) was held at reflux overnight. After cooling down to room 15 temperature the organic phase was washed with water, dried over MgSO 4 and concentrated under reduced pressure. The crude product was dissolved in THF (12.0 ml) and thioacetamide (6.75 mmol) and solid NaHCO 3 (6.07 mmol) were added. The mixture was heated to reflux for 6 h and then it was filtered. The solvent was removed and the crude product purified by column chromatography using 20 heptane/ethyl acetate as eluent system to provide 2-methyl-4-p-tolyl-thiazole 5-carboxylic acid methyl ester (2.67 mmol). 2-Methyl-4-p-tolyl-thiazole-5-carboxylic acid methyl ester (2.67 mmol) and solid KOH (5.35 mmol) were dissolved in ethanol (1.04 mL) and water (0.26 mL) and heated under reflux for 3 hours. After cooling, the solvent was evaporated under reduced 25 pressure and ice water was added to the residue, followed by washing with hexane. The aqueous layer was acidified with 1N aq. HCI and the crystals thus precipitated were collected by filtration, washed with water and then dried to provide 2-methyl 4-p-tolyl-thiazole-5-carboxylic acid. LC-MS: tR = 0.83 min; [M+H]* = 234.02.
    WO 2008/117241 PCT/IB2008/051110 47 A.2 Synthesis of 2-methyl-oxazole-4-carboxylic acid derivatives A.2.1 Synthesis of 2-acetylamino-3-oxo-propionic acid methyl ester derivatives (general procedure) 0 0 0 0 NaNO2 ' DJA' j H D 0~ N CH 3 COOH, HgCl 2 HN NOH 5 A solution of the respective 3-oxo-propionic acid methyl ester derivative (4.8 mmol, 1.0 eq.) in glacial acetic acid (1.9 mL) was cooled to 100C and at this temperature was added a solution of NaNO 2 (5.6 mmol, 1.16 eq.) in water (0.68 mL). After the addition was complete (15 min), the solution was allowed to warm to room temperature and stirred for 2 h. Then the solution was poured into water (10 mL) and after a few minutes 10 crystals begun to appear. This suspension was cooled in an ice-bath and crystals were collected by filtration. The cake was washed several times with cold water and the water was removed by the azeotrope of toluene-water in vacuo to give 2-hydroxyimino 3-oxo-propionic acid methyl ester derivatives which were dissolved in a mixture of acetic anhydride (1.375 mL) and glacial acetic acid (1.8 mL). To this solution was 15 added sodium acetate (0.296 mmol, 0.06 eq.) and HgCl 2 (0.01 mmol, 0.002 eq.). The mixture was refluxed for 1 h, then cooled to room temperature and filtered. The solid was rinsed with ether, the organic filtrate was recovered, washed 3 times with water and one time with 1 M aq. K 2
    CO
    3 The organic layer was dried over MgSO4, filtered and concentrated. The crude products were purified by flash chromatography to afford the 20 corresponding 2-acetylamino-3-oxo-propionic acid methyl ester derivatives. 2-Acetylamino-3-oxo-3-(3-trifluoromethyl-phenyl)-propionic acid methyl ester prepared according to general procedure A.2.1 from 3-oxo-(3-trifluoromethyl-phenyl) propionic acid methyl ester. 2-Acetylamino-3-oxo-3-m-tolyl-propionic acid methyl ester 25 prepared according to general procedure A.2.1 from 3-oxo-3-m-tolyl-propionic acid methyl ester. 2-Acetylamino-3-oxo-3-p-tolyl-propionic acid methyl ester prepared according to general procedure A.2.1 from 3-oxo-3-p-tolyl-propionic acid methyl ester. 30 2-Acetylamino-3-(4-fluoro-phenyl)-3-oxo-propionic acid methyl ester WO 2008/117241 PCT/IB2008/051110 48 prepared according to general procedure A.2.1 from 3-oxo-3-(4-fluoro-phenyl) propionic acid methyl ester. 2-Acetylamino-3-(4-methoxy-phenyl)-3-oxo-propionic acid methyl ester prepared according to general procedure A.2.1 from 3-oxo-3-(4-methoxy-phenyl) 5 propionic acid methyl ester. A.2.2 Synthesis of 2-methyl-oxazole-4-carboxylic acid derivatives (general procedure) 0 0 D D D SOCI 0 NaOH 0 HN 0~N 0N OH A solution of the respective 2-acetylamino-3-oxo-propionic acid methyl ester derivative 10 (0.63 mmol, 1.0 eq.) in chloroform (0.4 mL) was cooled to OC in an ice/NaCI bath. SOC1 2 (0.88 mmol, 1.4 eq.) was added to the stirred solution and the temperature was maintained at 00C for 30 minutes. Then the solution was stirred and refluxed for one hour. Another 0.25 eq. of SOC12 was added and the reaction mixture was refluxed for another hour. 15 The excess SOC12 was quenched with 1 M aq. K 2
    CO
    3 . The aqueous layer was extracted twice with ether. The combined organic phases were washed once with water and dried over MgSO 4 , filtered and concentrated yielding the corresponding 2-methyl-oxazole-4-carboxylic acid methyl ester derivative. The respective 2-methyl oxazole-4-carboxylic acid methyl ester derivative was dissolved in a mixture of ethanol 20 (0.7 ml) and 2N aq. NaOH (0.7 mL, 2.5 eq.). The mixture was stirred at RT for 2 hours. The reaction mixture was washed once with ether and this organic layer was discarded. The aqueous layer was then acidified with conc. HCI and extracted twice with ether. Both organic layers were combined, dried over MgSO 4 and concentrated in vacuo to afford the corresponding 2-methyl-oxazole-4-carboxylic acid derivatives. 25 2-Methyl-5-m-tolyl-oxazole-4-carboxylic acid prepared according to general procedure A.2.2 from 2-acetylamino-3-oxo-3-m-tolyl propionic acid methyl ester LC-MS: tR = 0.51 min; [M-H]* = 216.33. 2-Methyl-5-(3-trifluoromethyl-phenyl)-oxazole-4-carboxylic acid WO 2008/117241 PCT/IB2008/051110 49 prepared according to general procedure A.2.2 from 2-acetylamino-3-oxo-3 (3-trifluoromethyl-phenyl)-propionic acid methyl ester. LC-MS: tR = 0.55 min; [M-H]* = 270.24. 2-Methyl-5-p-tolyl-oxazole-4-carboxylic acid 5 prepared according to general procedure A.2.2 from 2-acetylamino-3-oxo-3-p-tolyl propionic acid methyl ester. LC-MS: tR = 0.55 min; [M-H]* = 216.34. 5-(4-Fluoro-phenyl)-2-methyl-oxazole-4-carboxylic acid prepared according to general procedure A.2.2 from 2-acetylamino-3-(4-fluoro-phenyl) 3-oxo-propionic acid methyl ester. LC-MS: tR = 0.49 min; [M-H]* = 220.30. 10 5-(4-Methoxy-phenyl)-2-methyl-oxazole-4-carboxylic acid prepared according to general procedure A.2.2 from 2-acetylamino-3-(4-methoxy phenyl)-3-oxo-propionic acid methyl ester. LC-MS: tR = 0.77 min; [M+H]* = 234.31. 5-(3-Methoxy-phenyl)-2-methyl-oxazole-4-carboxylic acid prepared according to general procedure A.2.2 from 2-acetylamino-3-(3-methoxy 15 phenyl)-3-oxo-propionic acid methyl ester. LC-MS: tR = 0.49 min; [M+H]* = 232.30. A.3 Biphenyl-2-carboxylic acid derivatives The following biphenyl-2-carboxylic acid derivatives are commercially available: Biphenyl-2-carboxylic acid; 4'-Methyl-biphenyl-2-carboxylic acid; 20 3'-Methyl-biphenyl-2-carboxylic acid; 3',4'-Dimethyl-biphenyl-2-carboxylic acid; 4'-Methoxy-biphenyl-2-carboxylic acid; 3'-Methoxy-biphenyl-2-carboxylic acid; 4'-Fluoro-biphenyl-2-carboxylic acid. 25 A.4 Synthesis of 4-{[acyl-amino]-methyl}-thiazolidine derivatives R 1 S NH 2 S H N-O R N + HOOC-R 1 N ON N O N H A.4.1 Synthesis of 4-{[acyl-amino]-methyl}-thiazolidine-3-carboxylic acid tert butyl ester derivatives (general procedure) WO 2008/117241 PCT/IB2008/051110 50 To a solution of the respective carboxylic acid R'COOH, wherein R 1 is as defined for formula (1), (4.6 mmol, 1.0 eq.), 4-aminomethyl-thiazolidine-3-carboxylic acid tert-butyl ester (4.6 mmol, 1.0 eq.) and DMAP (2.3 mmol, 1.0 eq.) in DCM (25 mL) was added under stirring solid EDC (4.7 mmol, 1.02 eq.). Stirring continued over night. Then sat. 5 aq. NaHCO 3 solution (6 mL) was added to the reaction mixture and stirring continued for 30 min. The organic phase was separated, the solvent was stripped off yielding the crude corresponding 4-{[acyl-amino]-methyl}-thiazolidine-3-carboxylic acid tert-butyl ester derivative. The following intermediates were synthesized according to general procedure A.4.1 10 from commercially available (R)-4-aminomethyl-thiazolidine-3-carboxylic acid tert-butyl ester and the respective carboxylic acid R'COOH, which is commercially available or synthesized according to methods described above: Intermediate Name [M+H]* tR (R)-4-{[(Benzofuran-4-carbonyl)-amino] 11 methyl}-thiazolidine-3-carboxylic acid tert-butyl 363.06 0.96 ester (R)-4-{[(6-Methyl-imidazo[2,1 -b]thiazole-5 12 carbonyl)-amino]-methyl}-thiazolidine-3- 383.03 0.81 carboxylic acid tert-butyl ester (R)-4-{[(1 -Methyl-1 H-indazole-3-carbonyl) 13 amino]-methyl}-thiazolidine-3-carboxylic acid 377.07 0.97 tert-butyl ester (R)-4-{[(2,3-Dihydro-benzo[1,4]dioxine-5 14 carbonyl)-amino]-methyl}-thiazolidine-3- 381.06 0.94 carboxylic acid tert-butyl ester 15 (R)-4-[(3-Bromo-benzoylamino)-methyl]- 402.81 1.00 thiazolidine-3-carboxylic acid tert-butyl ester (R)-4-{[(1 -Methyl-1 H-indole-2-carbonyl)-amino] 16 methyl}-thiazolidine-3-carboxylic acid tert-butyl 376.08 1.02 ester (R)-4-{[(4-Bromo-thiophene-2-carbonyl)-amino] 17 methyl}-thiazolidine-3-carboxylic acid tert-butyl 408.93 0.99 ester WO 2008/117241 PCT/IB2008/051110 51 (R)-4-{[(6-Bromo-pyridine-2-carbonyl)-amino] 18 methyl}-thiazolidine-3-carboxylic acid tert-butyl 403.97 0.98 ester A.4.2 Synthesis of 4-{[acyl-amino]-methyl}-thiazolidine derivatives The respective 4-{[acyl-amino]-methyl}-thiazolidine-3-carboxylic acid tert-butyl ester derivative (0.15 mmol, 1.0 eq.) was dissolved in a mixture of dry MeOH (0.33 mL) and 5 4 M HCI in dioxane (0.75 mL, 20 eq.). After shaking for 2 h the solvent was evaporated and the residue treated with dry MeOH (1 mL) followed by evaporation and drying under vacuo for 14 h to provide the corresponding 4-{[acyl-amino]-methyl}-thiazolidine derivative hydrochloride. Preparation of Examples (general procedure 1) 10 The corresponding thiazole-carboxylic acid derivative (Intermediate according to method A.1), 2-methyl-oxazole-4-carboxylic acid derivative (Intermediate according to method A.2), or biphenyl-2-carboxylic acid derivative (Intermediate according to method A.3), respectively, (0.15 mmol, 1.0 eq.) was dissolved in a mixture of dry DMF (1.0 mL) and DIPEA (0.58 mmol, 3.9 eq.) under stirring. Solid HATU 15 (0.15 mmol, 1.0 eq.) was added and stirring was allowed for 10 min. This solution was added to the respective crude 4-{[acyl-amino]-methyl}-thiazolidine derivative hydrochloride (Intermediate according to method A.4) and the mixture was stirred over night. The DMF was evaporated under vacuum and the crude product purified by prep. HPLC to provide the final compound. 20 The following Examples given in table 1 were synthesized according to the general procedure 1 given above: Table 1: Example Name [M+H]* tR Benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro 1 phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin-4- 482.03 0.97 ylmethyl}-amide WO 2008/117241 PCT/IB2008/051110 52 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid 2 {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4- 502.09 0.83 carbonyl]-thiazolidin-4-ylmethyl}-amide 2,3-Dihydro-benzo[1,4]dioxine-5-carboxylic acid 3 {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4- 500.12 0.96 carbonyl]-thiazolidin-4-ylmethyl}-amide 1-Methyl-1 H-indazole-3-carboxylic acid {(R)-3-[5 4 (4-fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]- 496.12 0.97 thiazolidin-4-ylmethyl}-amide Benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5 5 phenyl-thiazole-4-carbonyl)-thiazolidin-4-ylmethyl]- 464.13 0.96 amide Benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-p 6 tolyl-thiazole-4-carbonyl)-thiazolidin-4-ylmethyl]- 478.13 0.99 amide Benzofuran-4-carboxylic acid {(R)-3-[2-methyl-5-(4 7 trifluoromethyl-phenyl)-thiazole-4-carbonyl]- 532.06 1.02 thiazolidin-4-ylmethyl}-amide Benzofuran-4-carboxylic acid {(R)-3-[5-(3-fluoro 8 phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin-4- 482.11 0.98 ylmethyl}-amide Benzofuran-4-carboxylic acid {(R)-3-[5-(3,4 9 dimethyl-phenyl)-2-methyl-thiazole-4-carbonyl]- 492.14 1.02 thiazolidin-4-ylmethyl}-amide Benzofuran-4-carboxylic acid {(R)-3-[5-(2-fluoro 10 phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin-4- 482.10 0.98 ylmethyl}-amide Benzofuran-4-carboxylic acid {(R)-3-[5-(3-methoxy 11 phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin-4- 494.12 0.97 ylmethyl}-amide Benzofuran-4-carboxylic acid {(R)-3-[2-methyl-5-(3 12 trifluoromethyl-phenyl)-thiazole-4-carbonyl]- 532.07 1.03 thiazolidin-4-ylmethyl}-amide WO 2008/117241 PCT/IB2008/051110 53 Benzofuran-4-carboxylic acid {(R)-3-[5-(3,4 13 difluoro-phenyl)-2-methyl-thiazole-4-carbonyl]- 500.11 0.99 thiazolidin-4-ylmethyl}-amide Benzofuran-4-carboxylic acid {(R)-3-[5-(2-methoxy 14 phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin-4- 494.11 0.97 ylmethyl}-amide Benzofuran-4-carboxylic acid {(R)-3-[2-methyl-5-(3 15 trifluoromethyl-phenyl)-oxazole-4-carbonyl]- 516.10 1.01 thiazolidin-4-ylmethyl}-amide Benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-p 16 tolyl-oxazole-4-carbonyl)-thiazolidin-4-ylmethyl]- 462.15 0.98 amide Benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro 17 phenyl)-2-methyl-oxazole-4-carbonyl]-thiazolidin-4- 466.14 0.96 ylmethyl}-amide Benzofuran-4-carboxylic acid [(R)-3-(2-methyl-4-p 18 tolyl-thiazole-5-carbonyl)-thiazolidin-4-ylmethyl]- 478.14 0.98 amide Benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-m 19 tolyl-oxazole-4-carbonyl)-thiazolidin-4-ylmethyl]- 462.15 0.98 amide Benzofuran-4-carboxylic acid {(R)-3-[5-(3,4 20 dichloro-phenyl)-2-methyl-thiazole-4-carbonyl]- 532.02 1.04 thiazolidin-4-ylmethyl}-amide Benzofuran-4-carboxylic acid {(R)-3-[5-(4-methoxy 21 phenyl)-2-methyl-oxazole-4-carbonyl]-thiazolidin-4- 478.15 0.96 ylmethyl}-amide 22 Benzofuran-4-carboxylic acid [(R)-3-(4'-methyl- 457.17 1.03 biphenyl-2-carbonyl)-thiazolidin-4-ylmethyl]-amide 23 Benzofuran-4-carboxylic acid [(R)-3-(3',4'-dimethyl- 471.18 1.05 biphenyl-2-carbonyl)-thiazolidin-4-ylmethyl]-amide 24 Benzofuran-4-carboxylic acid [(R)-3-(3'-methyl- 457.17 1.03 biphenyl-2-carbonyl)-thiazolidin-4-ylmethyl]-amide WO 2008/117241 PCT/IB2008/051110 54 25 Benzofuran-4-carboxylic acid [(R)-3-(3'-methoxy- 473.17 1.00 biphenyl-2-carbonyl)-thiazolidin-4-ylmethyl]-amide 26 Benzofuran-4-carboxylic acid [(R)-3-(4'-fluoro- 461.14 1.01 biphenyl-2-carbonyl)-thiazolidin-4-ylmethyl]-amide 27 Benzofuran-4-carboxylic acid [(R)-3-(4'-methoxy- 473.15 1.00 biphenyl-2-carbonyl)-thiazolidin-4-ylmethyl]-amide 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid 28 [(R)-3-(2-methyl-5-phenyl-thiazole-4-carbonyl)- 484.10 0.81 thiazolidin-4-ylmethyl]-amide 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid 29 [(R)-3-(2-methyl-5-p-tolyl-thiazole-4-carbonyl)- 498.10 0.85 thiazolidin-4-ylmethyl]-amide 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid 30 {(R)-3-[2-methyl-5-(4-trifluoromethyl-phenyl)- 552.06 0.91 thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid 31 {(R)-3-[5-(3-fluoro-phenyl)-2-methyl-thiazole-4- 502.03 0.83 carbonyl]-thiazolidin-4-ylmethyl}-amide 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid 32 {(R)-3-[5-(3,4-dimethyl-phenyl)-2-methyl-thiazole-4- 512.10 0.88 carbonyl]-thiazolidin-4-ylmethyl}-amide 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid 33 {(R)-3-[5-(2-fluoro-phenyl)-2-methyl-thiazole-4- 502.03 0.83 carbonyl]-thiazolidin-4-ylmethyl}-amide 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid 34 {(R)-3-[5-(3-methoxy-phenyl)-2-methyl-thiazole-4- 514.08 0.83 carbonyl]-thiazolidin-4-ylmethyl}-amide 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid 35 {(R)-3-[2-methyl-5-(3-trifluoromethyl-phenyl)- 552.06 0.90 thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid 36 {(R)-3-[5-(3,4-difluoro-phenyl)-2-methyl-thiazole-4- 520.05 0.86 carbonyl]-thiazolidin-4-ylmethyl}-amide WO 2008/117241 PCT/IB2008/051110 55 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid 37 {(R)-3-[5-(2-methoxy-phenyl)-2-methyl-thiazole-4- 514.10 0.83 carbonyl]-thiazolidin-4-ylmethyl}-amide 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid 38 {(R)-3-[2-methyl-5-(3-trifluoromethyl-phenyl)- 536.08 0.89 oxazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid 39 [(R)-3-(2-methyl-5-p-tolyl-oxazole-4-carbonyl)- 482.14 0.80 thiazolidin-4-ylmethyl]-amide 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid 40 {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-oxazole-4- 486.11 0.80 carbonyl]-thiazolidin-4-ylmethyl}-amide 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid 41 [(R)-3-(2-methyl-4-p-tolyl-thiazole-5-carbonyl)- 498.13 0.83 thiazolidin-4-ylmethyl]-amide 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid 42 [(R)-3-(2-methyl-5-m-tolyl-oxazole-4-carbonyl)- 482.14 0.81 thiazolidin-4-ylmethyl]-amide 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid 43 {(R)-3-[5-(3,4-dichloro-phenyl)-2-methyl-thiazole-4- 551.99 0.91 carbonyl]-thiazolidin-4-ylmethyl}-amide 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid 44 {(R)-3-[5-(4-methoxy-phenyl)-2-methyl-oxazole-4- 498.11 0.78 carbonyl]-thiazolidin-4-ylmethyl}-amide 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid 45 [(R)-3-(4'-methyl-biphenyl-2-carbonyl)-thiazolidin-4- 477.13 0.90 ylmethyl]-amide 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid 46 [(R)-3-(3',4'-dimethyl-biphenyl-2-carbonyl)- 491.16 0.93 thiazolidin-4-ylmethyl]-amide 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid 47 [(R)-3-(3'-methyl-biphenyl-2-carbonyl)-thiazolidin-4- 477.15 0.90 ylmethyl]-amide WO 2008/117241 PCT/IB2008/051110 56 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid 48 [(R)-3-(3'-methoxy-biphenyl-2-carbonyl)-thiazolidin- 493.13 0.87 4-ylmethyl]-amide 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid 49 [(R)-3-(4'-fluoro-biphenyl-2-carbonyl)-thiazolidin-4- 481.12 0.88 ylmethyl]-amide 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid 50 [(R)-3-(4'-methoxy-biphenyl-2-carbonyl)-thiazolidin- 493.13 0.87 4-ylmethyl]-amide 1-Methyl-1 H-indazole-3-carboxylic acid [(R)-3-(2 51 methyl-5-phenyl-thiazole-4-carbonyl)-thiazolidin-4- 478.15 0.96 ylmethyl]-amide 1-Methyl-1 H-indazole-3-carboxylic acid [(R)-3-(2 52 methyl-5-p-tolyl-thiazole-4-carbonyl)-thiazolidin-4- 492.15 0.99 ylmethyl]-amide 1-Methyl-1 H-indazole-3-carboxylic acid {(R)-3-[2 53 methyl-5-(4-trifluoromethyl-phenyl)-thiazole-4- 546.11 1.02 carbonyl]-thiazolidin-4-ylmethyl}-amide 1-Methyl-1 H-indazole-3-carboxylic acid {(R)-3-[5 54 (3-fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]- 496.11 0.97 thiazolidin-4-ylmethyl}-amide 1-Methyl-1 H-indazole-3-carboxylic acid {(R)-3-[5 55 (3,4-dimethyl-phenyl)-2-methyl-thiazole-4- 506.15 1.02 carbonyl]-thiazolidin-4-ylmethyl}-amide 1-Methyl-1 H-indazole-3-carboxylic acid {(R)-3-[5 56 (2-fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]- 496.12 0.98 thiazolidin-4-ylmethyl}-amide 1-Methyl-1 H-indazole-3-carboxylic acid {(R)-3-[5 57 (3-methoxy-phenyl)-2-methyl-thiazole-4-carbonyl]- 508.14 0.97 thiazolidin-4-ylmethyl}-amide 1-Methyl-1 H-indazole-3-carboxylic acid {(R)-3-[2 58 methyl-5-(3-trifluoromethyl-phenyl)-thiazole-4- 546.11 1.02 carbonyl]-thiazolidin-4-ylmethyl}-amide WO 2008/117241 PCT/IB2008/051110 57 1-Methyl-1 H-indazole-3-carboxylic acid {(R)-3-[5 59 (3,4-difluoro-phenyl)-2-methyl-thiazole-4-carbonyl]- 514.11 0.98 thiazolidin-4-ylmethyl}-amide 1-Methyl-1 H-indazole-3-carboxylic acid {(R)-3-[5 60 (2-methoxy-phenyl)-2-methyl-thiazole-4-carbonyl]- 508.14 1.00 thiazolidin-4-ylmethyl}-amide 1-Methyl-1 H-indazole-3-carboxylic acid {(R)-3-[2 61 methyl-5-(3-trifluoromethyl-phenyl)-oxazole-4- 530.13 0.99 carbonyl]-thiazolidin-4-ylmethyl}-amide 1-Methyl-1 H-indazole-3-carboxylic acid [(R)-3-(2 62 methyl-5-p-tolyl-oxazole-4-carbonyl)-thiazolidin-4- 476.19 0.96 ylmethyl]-amide 1-Methyl-1 H-indazole-3-carboxylic acid {(R)-3-[5 63 (4-fluoro-phenyl)-2-methyl-oxazole-4-carbonyl]- 480.17 0.93 thiazolidin-4-ylmethyl}-amide 1-Methyl-1 H-indazole-3-carboxylic acid [(R)-3-(2 64 methyl-4-p-tolyl-thiazole-5-carbonyl)-thiazolidin-4- 492.14 0.98 ylmethyl]-amide 1-Methyl-1 H-indazole-3-carboxylic acid [(R)-3-(2 65 methyl-5-m-tolyl-oxazole-4-carbonyl)-thiazolidin-4- 476.18 0.96 ylmethyl]-amide 1-Methyl-1 H-indazole-3-carboxylic acid {(R)-3-[5 66 (3,4-dichloro-phenyl)-2-methyl-thiazole-4-carbonyl]- 546.03 1.03 thiazolidin-4-ylmethyl}-amide 1-Methyl-1 H-indazole-3-carboxylic acid {(R)-3-[5 67 (4-methoxy-phenyl)-2-methyl-oxazole-4-carbonyl]- 492.16 0.93 thiazolidin-4-ylmethyl}-amide 1-Methyl-1 H-indazole-3-carboxylic acid [(R)-3-(4' 68 methyl-biphenyl-2-carbonyl)-thiazolidin-4-ylmethyl]- 471.19 1.03 amide 1-Methyl-1 H-indazole-3-carboxylic acid [(R)-3 69 (3',4'-dimethyl-biphenyl-2-carbonyl)-thiazolidin-4- 485.2 1.05 ylmethyl]-amide WO 2008/117241 PCT/IB2008/051110 58 1-Methyl-1 H-indazole-3-carboxylic acid [(R)-3-(3' 70 methyl-biphenyl-2-carbonyl)-thiazolidin-4-ylmethyl]- 471.20 1.03 amide 1-Methyl-1 H-indazole-3-carboxylic acid [(R)-3-(3' 71 methoxy-biphenyl-2-carbonyl)-thiazolidin-4- 487.18 1.00 ylmethyl]-amide 1-Methyl-1 H-indazole-3-carboxylic acid [(R)-3-(4' 72 fluoro-biphenyl-2-carbonyl)-thiazolidin-4-ylmethyl]- 475.17 1.01 amide 1-Methyl-1 H-indazole-3-carboxylic acid [(R)-3-(4' 73 methoxy-biphenyl-2-carbonyl)-thiazolidin-4- 487.18 1.01 ylmethyl]-amide 3-Bromo-N-{(R)-3-[5-(4-fluoro-phenyl)-2-methyl 74 thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}- 519.99 1.00 benzamide 1-Methyl-1 H-indole-2-carboxylic acid {(R)-3-[5-(4 75 fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]- 495.14 1.02 thiazolidin-4-ylmethyl}-amide 4-Bromo-thiophene-2-carboxylic acid {(R)-3-[5-(4 76 fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]- 525.95 0.99 thiazolidin-4-ylmethyl}-amide 6-Bromo-pyridine-2-carboxylic acid {(R)-3-[5-(4 77 fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]- 521.00 0.98 thiazolidin-4-ylmethyl}-amide 2,3-Dihydro-benzo[1,4]dioxine-5-carboxylic acid 78 [(R)-3-(biphenyl-2-carbonyl)-thiazolidin-4-ylmethyl]- 461.17 0.98 amide 79 Benzofuran-4-carboxylic acid [(R)-3-(biphenyl-2- 443.06 1.00 carbonyl)-thiazolidin-4-ylmethyl]-amide 80 N-[(R)-3-(Biphenyl-2-carbonyl)-thiazolidin-4- 481.06 1.02 ylmethyl]-3-bromo-benzamide 81 1 -Methyl-1 H-indole-2-carboxylic acid [(R)-3- 456.19 1.04 (biphenyl-2-carbonyl)-thiazolidin-4-ylmethyl]-amide WO 2008/117241 PCT/IB2008/051110 59 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid 82 [(R)-3-(biphenyl-2-carbonyl)-thiazolidin-4-ylmethyl]- 463.15 0.87 amide 83 1 -Methyl-1 H-indazole-3-carboxylic acid [(R)-3- 457.18 1.00 (biphenyl-2-carbonyl)-thiazolidin-4-ylmethyl]-amide 84 4-Bromo-thiophene-2-carboxylic acid [(R)-3- 487.01 1.02 (biphenyl-2-carbonyl)-thiazolidin-4-ylmethyl]-amide 85 6-Bromo-pyridine-2-carboxylic acid [(R)-3- 482.03 1.01 (biphenyl-2-carbonyl)-thiazolidin-4-ylmethyl]-amide A.5 Synthesis of 3-acyl-(4-aminomethyl)-thiazolidine derivatives T H F A F A-CO 2 1 N H 2 C F 3 CO 2 Et S N N F3 TF y, 3 N F 3 A -CO2H , 3 N H 2 N-- - N LN N 0O O H.CF 3
    CO
    2 H /6O 5 A.5.1 Synthesis of 4-[(2,2,2-trifluoro-acetylamino)-methyl]-thiazolidine-3 carboxylic acid tert-butyl ester To a solution of (R)-4-Aminomethyl-thiazolidine-3-carboxylic acid tert-butyl ester (19 mmol) in dry THF (56 mL) was added slowly ethyl trifluoroacetate (22.8 mmol, 1.2 eq)). 10 The reaction mixture was stirred at rt for 20 h and concentrated to yield the crude title compound which was used for the next step without further purification. LC-MS: tR = 0.94 min; [M+H]* = 315.04. A.5.2 Synthesis of 2,2,2-trifluoro-N-thiazolidin-4-ylmethyl-acetamide (trifluoroacetic acid salt) 15 To a cold (OC) solution of 4-[(2,2,2-trifluoro-acetylamino)-methyl]-thiazolidine-3 carboxylic acid tert-butyl ester (19 mmol) in dry DCM (10 mL) was added dropwise TFA (133 mmol, 7 eq). The reaction mixture was stirred for 20 h and concentrated in vacuo to give the title compound. LC-MS: tR = 0.24 min; [M+H]* = 214.99.
    WO 2008/117241 PCT/IB2008/051110 60 A.5.3 Synthesis of 3-acyl-(4-aminomethyl)-thiazolidine derivatives (general procedure) A solution of the respective carboxylic acid A-COOH (4.6 mmol, 1 eq), TBTU (4.6 mmol, 1 eq), DIPEA (23 mmol, 5 eq) in dry DMF (15 mL) was stirred at rt for 30 min. 5 Then, was added 2,2,2-trifluoro-N-thiazolidin-4-ylmethyl-acetamide (trifluoroacetic acid salt) (4.6 mmol, 1.0 eq.) in dry DMF (0.5 mL). Stirring was continued over night. The reaction mixture was partitioned between sat. aq. NaHCO 3 solution/ EtOAc. The organic phase was washed with brine, dried (MgSO 4 ), filtered and concentrated to give the corresponding N-(thiazolidin-4-ylmethyl)-2,2,2-trifluoro-acetamide derivative which 10 was used for the next step without further purification. To a solution of the above product (4.6 mmol) in dry MeOH (32 mL) was added sat. K 2
    CO
    3 (32 mL). The reaction mixture was stirred at rt for 20 h. Diethyl ether was added, and the organic phase was washed with 25% HCl, 1 N HCI. The aqueous phase was basified with 30% NaOH and extracted with DCM. The combined organic extracts were washed with brine, dried 15 (MgSO4), filtered and concentrated to give the corresponding 3-acyl-(4-aminomethyl) thiazolidine derivative which was used for the next step without further purification. The following intermediates were synthesized according to general procedure A.5.3 from commercially available (R)-4-aminomethyl-thiazolidine-3-carboxylic acid tert-butyl ester and the respective carboxylic acid A-COOH, which is commercially available or 20 synthesized according to methods described above: Intermediate Name [M+H]* tR 11 (4-Aminomethyl-thiazolidin-3-yl)-(2-methyl-5-m- 334.11 0.73 tolyl-thiazol-4-yl)-methanone 12 (4-Aminomethyl-thiazolidin-3-yl)-[5-(4-fluoro- 337.99 0.72 phenyl)-2-methyl-thiazol-4-yl]-methanone Preparation of Examples (general procedure 2) The corresponding acid R'COOH (0.15 mmol, 1.0 eq.) was dissolved in a mixture of dry DMF (1.0 mL) and DIPEA (0.75 mmol, 5 eq.) under stirring. Solid TBTU 25 (0.15 mmol, 1.0 eq.) was added and stirring was allowed for 15 min. Then was added a solution of the respective 3-acyl-(4-aminomethyl)-thiazolidine derivative (0.15 mmol, 1 WO 2008/117241 PCT/IB2008/051110 61 eq) (Intermediate according to method A.5.3) in dry DMF (0.5 mL) and the mixture was stirred over night. The DMF was evaporated under vacuum and the crude product purified by prep. HPLC to provide the final compound. The following Examples given in table 2 were synthesized according to the general 5 procedure 2 given above: Table 2: Example Name [M+H]* tR Naphthalene-1-carboxylic acid {(R)-3-[5-(4-fluoro 86 phenyl)-2-methyl-thiazole-4-carbonyl] thiazolidin-4- 492.14 1.01 ylmethyl}-amide 5-tert-Butyl-2-methyl-2H-pyrazole-3-carboxylic acid {(R) 87 3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]- 502.17 0.99 thiazolidin-4-ylmethyl}-amide 2,3-Dihydro-benzofuran-7-carboxylic acid {(R)-3[5-(4 88 fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin- 484.14 0.99 4-ylmethyl}-amide 2,4-Dimethyl-thiazole-5-carboxylic acid {(R)-3-[5-(4 89 fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin- 477.09 0.89 4-ylmethyl}-amide N-{(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4 90 carbonyl]-thiazolidin-4-ylmethyl}-3-nitro-benzamide 487.09 0.97 Benzo[b]thiophene-2-carboxylic acid {(R)-3-[5-(4-fluoro 91 phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin-4- 498.01 1.01 ylmethyl}-amide 2-Methyl-benzofu ran-4-carboxylic acid [(R)-3-(2-methyl 92 5-m-tolyl-thiazole-4-carbonyl)-thiazoidin-4-ylmethyl]- 492.11 1.02 amide 2-Methyl-benzooxazole-4-carboxylic acid [(R)-3-(2 93 methyl-5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4- 493.11 1 ylmethyl]-amide Imidazo[1,2-a]pyridine-3-carboxylic acid [(R)-3-(2 94 methyl-5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4- 478.13 0.78 ylmethyl]-amide WO 2008/117241 PCT/IB2008/051110 62 2-Methyl-imidazo[1,2-a]pyridine-3-carboxylic acid [(R)-3 95 (2-methyl-5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4- 492.12 0.78 ylmethyl]-amide 2,3-Dimethyl-benzofuran-4-carboxylic acid [(R)-3-(2 96 methyl-5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4- 506.03 1.04 ylmethyl]-amide 3,4-Dihydro-2H-benzo[1,4]oxazine-5-carboxylic acid 97 [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl)- 495.14 1 thiazolidin-4-ylmethyl]-amide 4-Methyl-3,4-Dihydro-2H-benzo[1,4]oxazine-5 98 carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4- 509.13 0.97 carbonyl)-thiazolidin-4-ylmethyl]-amide 99 Chroman-5-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl- 494.12 0.99 thiazole-4-carbonyl)-thiazolidin-4-ylmethyl]-amide 100 Chroman-8-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl- 494.13 1.01 thiazole-4-carbonyl)-thiazolidin-4-ylmethyl]-amide 3,4-Dihydro-2H-benzo[1,4]oxazine-8-carboxylic acid 101 [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl)- 495.12 0.92 thiazolidin-4-ylmethyl]-amide 4-Methyl-3,4-Dihydro-2H-benzo[1,4]oxazine-8 102 carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4- 509.13 0.99 carbonyl)-thiazolidin-4-ylmethyl]-amide Benzo[d]isoxazole-3-carboxylic acid [(R)-3-(2-methyl-5 103 m-tolyl-thiazole-4-carbonyl)-thiazolidin-4-ylmethyl]- 479.1 0.99 amide Benzo[d]isothiazole-3-carboxylic acid [(R)-3-(2-methyl 104 5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4-ylmethyl]- 495.06 1.04 amide 1-Methyl-1 H-indazole-3-carboxylic acid [(R)-3-(2-methyl 105 5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4-ylmethyl]- 492.11 0.99 amide 2,3-Dihydro-benzofuran-4-carboxylic acid [(R)-3-(2 106 methyl-5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4- 480.12 0.98 ylmethyl]-amide WO 2008/117241 PCT/IB2008/051110 63 2,3-Dihydro-benzo[1,4]dioxine-5-carboxylic acid [(R)-3 107 (2-methyl-5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4- 496.1 0.97 ylmethyl]-amide 2-Methyl-benzofu ran-4-carboxylic acid {(R)-3-[5-(4 108 fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin- 496.09 1 4-ylmethyl}-amide Imidazo[1,2-a]pyridine-3-carboxylic acid {(R)-3-[5-(4 109 fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin- 482.04 0.76 4-ylmethyl}-amide 2-Methyl-imidazo[1,2-a]pyridine-3-carboxylic acid {(R)-3 110 [5-(4-fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]- 496.09 0.75 thiazolidin-4-ylmethyl}-amide 2,3-Dimethyl-benzofuran-4-carboxylic acid {(R)-3-[5-(4 111 fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin- 510.1 1.02 4-ylmethyl}-amide 3,4-Dihydro-2H-benzo[1,4]oxazine-5-carboxylic acid 112 {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4- 499.02 0.98 carbonyl]-thiazolidin-4-ylmethyl}-amide 4-Methyl-3,4-dihydro-2H-benzo[1,4]oxazine-5-carboxylic 113 acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4- 513.1 0.94 carbonyl]-thiazolidin-4-ylmethyl}-amide Chroman-5-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2 114 methyl-thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}- 498.05 0.97 amide Chroman-8-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2 115 methyl-thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}- 498.02 0.98 amide 3,4-Dihydro-2H-benzo[1,4]oxazine-8-carboxylic acid 116 {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4- 499.01 0.89 carbonyl]-thiazolidin-4-ylmethyl}-amide 4-Methyl-3,4-dihydro-2H-benzo[1,4]oxazine-8-carboxylic 117 acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4- 513.12 0.96 carbonyl]-thiazolidin-4-ylmethyl}-amide WO 2008/117241 PCT/IB2008/051110 64 2,3-Dihydro-benzofuran-4-carboxylic acid {(R)-3-[5-(4 118 fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin- 484.09 0.96 4-ylmethyl}-amide 119 Benzooxazole-7-carboxylic acid [(R)-3-(2-methyl-5-m- 479.11 0.92 tolyl-thiazole-4-carbonyl)-thiazolidin-4-ylmethyl]-amide Benzooxazole-7-carboxylic acid {(R)-3-[5-(4-fluoro 120 phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin-4- 482.98 0.89 ylmethyl}-amide 2-Methyl-benzooxazole-7-carboxylic acid [(R)-3-(2 121 methyl-5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4- 493.11 0.94 ylmethyl]-amide 2-Methyl-benzooxazole-7-carboxylic acid {(R)-3-[5-(4 122 fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin- 497.08 0.97 4-ylmethyl}-amide 123 Benzothiazole-7-carboxylic acid [(R)-3-(2-methyl-5-m- 495.07 0.97 tolyl-thiazole-4-carbonyl)-thiazolidin-4-ylmethyl]-amide Benzothiazole-7-carboxylic acid {(R)-3-[5-(4-fluoro 124 phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin-4- 498.06 0.95 ylmethyl}-amide 7-Ch loro-benzofuran-4-carboxylic acid [(R)-3-(2-methyl 125 5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4-ylmethyl]- 512.06 1.04 amide 7-Ch loro-benzofu ran-4-carboxylic acid {(R)-3-[5-(4 126 fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin- 516.02 1.01 4-ylmethyl}-amide 7-Fluoro-benzofuran-4-carboxylic acid [(R)-3-(2-methyl 127 5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4-ylmethyl]- 496.08 1.01 amide 7-Fluoro-benzofuran-4-carboxylic acid {(R)-3-[5-(4 128 fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin- 500.06 0.99 4-ylmethyl}-amide Pyrrolo[2,1 -b]thiazole-7-carboxylic acid [(R)-3-(2-methyl 129 5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4-ylmethyl]- 483.08 0.94 amide WO 2008/117241 PCT/IB2008/051110 65 Pyrrolo[2,1 -b]thiazole-7-carboxylic acid {(R)-3-[5-(4 130 fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin- 487.04 0.91 4-ylmethyl}-amide 6-Methyl-pyrrolo[2,1-b]thiazole-7-carboxylic acid [(R)-3 131 (2-methyl-5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4- 497.07 0.96 ylmethyl]-amide 6-Methyl-pyrrolo[2,1 -b]thiazole-7-carboxylic acid {(R)-3 132 [5-(4-fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]- 501.06 0.94 thiazolidin-4-ylmethyl}-amide 7-Chloro-2-methoxy-2,3-dihydro-benzofuran-4 133 carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4- 544.06 1.01 carbonyl)-thiazolidin-4-ylmethyl]-amide 7-Chloro-2-methoxy-2,3-dihydro-benzofuran-4 134 carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl- 548.05 1.04 thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide 2-Chloro-benzothiazole-4-carboxylic acid [(R)-3-(2 135 methyl-5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4- 529.03 1.04 ylmethyl]-amide 2-Chloro-benzothiazole-4-carboxylic acid {(R)-3-[5-(4 136 fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin- 532.99 1.02 4-ylmethyl}-amide 137 Benzothiazole-4-carboxylic acid [(R)-3-(2-methyl-5-m- 494.93 0.9 tolyl-thiazole-4-carbonyl)-thiazolidin-4-ylmethyl]-amide Benzothiazole-4-carboxylic acid {(R)-3-[5-(4-fluoro 138 phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin-4- 499.07 0.88 ylmethyl}-amide Benzo[1,2,5]thiadiazole-4-carboxylic acid [(R)-3-(2 139 methyl-5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4- 496.12 1.01 ylmethyl]-amide Benzo[1,2,5]thiadiazole-4-carboxylic acid {(R)-3-[5-(4 140 fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin- 500.1 0.99 4-ylmethyl}-amide 7-Trifl uoromethyl-benzofuran-4-carboxylic acid [(R)-3 141 (2-methyl-5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4- 546.08 1.07 ylmethyl]-amide WO 2008/117241 PCT/IB2008/051110 66 7-Trifl uoromethyl-benzofuran-4-carboxylic acid {(R)-3 142 [5-(4-fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]- 550.15 1.04 thiazolidin-4-ylmethyl}-amide 3-Methyl-benzofu ran-4-carboxylic acid [(R)-3-(2-methyl 143 5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4-ylmethyl]- 492.17 1.02 amide 3-Methyl-benzofu ran-4-carboxylic acid {(R)-3-[5-(4 144 fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin- 496.14 1 4-ylmethyl}-amide Benzo[2,1,3]oxadiazole-4-carboxylic acid [(R)-3-(2 145 methyl-5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4- 480.11 0.98 ylmethyl]-amide Benzo[2,1,3]oxadiazole-4-carboxylic acid {(R)-3-[5-(4 146 fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin- 484.12 0.89 4-ylmethyl}-amide 2-Hydroxymethyl-benzofu ran-4-carboxylic acid [(R)-3 147 (2-methyl-5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4- 508.17 0.91 ylmethyl]-amide 2-Hydroxymethyl-benzofu ran-4-carboxylic acid {(R)-3 148 [5-(4-fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]- 512.17 0.89 thiazolidin-4-ylmethyl}-amide 2-Fluoro-benzofuran-4-carboxylic acid [(R)-3-(2-methyl 149 5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4-ylmethyl]- 496.14 1.03 amide 5-Ch loro-2-methyl-benzofu ran-4-carboxylic acid {(R)-3 150 [5-(4-fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]- 526.11 1.05 thiazolidin-4-ylmethyl}-amide 7-Ch loro-2-methyl-benzofu ran-4-carboxylic acid [(R)-3 151 (2-methyl-5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4- 526.14 1.07 ylmethyl]-amide 7-Fluoro-2-methyl-benzofu ran-4-carboxylic acid [(R)-3 152 (2-methyl-5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4- 510.12 1.05 ylmethyl]-amide WO 2008/117241 PCT/IB2008/051110 67 2-Methyl-7-trifluoromethyl-benzofuran-4-carboxylic acid 153 [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl)- 560.23 1.09 thiazolidin-4-ylmethyl]-amide 6-Ch loro-2-methyl-benzofu ran-4-carboxylic acid [(R)-3 154 (2-methyl-5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4- 526.12 1.08 ylmethyl]-amide 6-Fluoro-2-methyl-benzofu ran-4-carboxylic acid [(R)-3 155 (2-methyl-5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4- 510.14 1.06 ylmethyl]-amide 2-Methyl-6-trifluoromethyl-benzofuran-4-carboxylic acid 156 [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl)- 560.23 1.1 thiazolidin-4-ylmethyl]-amide 5-Ch loro-2-methyl-benzofu ran-4-carboxylic acid [(R)-3 157 (2-methyl-5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4- 530.18 1.08 ylmethyl]-amide 7-Ch loro-2-methyl-benzofu ran-4-carboxylic acid {(R)-3 158 [5-(4-fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]- 530.18 1.08 thiazolidin-4-ylmethyl}-amide 7-Fluoro-2-methyl-benzofuran-4-carboxylic acid {(R)-3 159 [5-(4-fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]- 514.19 1.06 thiazolidin-4-ylmethyl}-amide 2-Methyl-7-trifluoromethyl-benzofuran-4-carboxylic acid 160 {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4- 564.22 1.1 carbonyl]-thiazolidin-4-ylmethyl}-amide 6-Ch loro-2-methyl-benzofu ran-4-carboxylic acid {(R)-3 161 [5-(4-fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]- 530.18 1.09 thiazolidin-4-ylmethyl}-amide 6-Fluoro-2-methyl-benzofuran-4-carboxylic acid {(R)-3 162 [5-(4-fluoro-phenyl)-2-methyl-thiazole-4-carbonyl]- 514.18 1.07 thiazolidin-4-ylmethyl}-amide 2-Methyl-6-trifluoromethyl-benzofuran-4-carboxylic acid 163 {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4- 564.21 1.08 carbonyl]-thiazolidin-4-ylmethyl}-amide WO 2008/117241 PCT/IB2008/051110 68 Example 164: 2-Trifluoromethyl-benzofuran-4-carboxylic acid [3-(2-methyl-5-m-tolyI-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide a) 2-lodo-benzofuran-4-carboxylic acid 5 To a cold (-78 0C) solution of benzofuran-4-carboxylic acid (100 mg) in dry diethyl ether (1.2 mL), was added dropwise tert-butyl lithium (1.7 M in pentane, 0.8 mL, 2.2 eq). The reaction mixture was stirred at -780C for 30 min. under nitrogen, then a solution of iodine (172.2 mg, 1.2 eq) in ether (1.9 mL) was added dropwise. The reaction was stirred at -780C for 30 min. and allowed to warm to rt. The reaction mixture was 10 partitioned between sat. NH 4 CI and diethyl ether, the aqueous phase was extracted once again with diethyl ether. The combined organic extracts were washed with sat. sodium thiosulfate, water, dried (Na 2
    SO
    4 ), filtered and concentrated to yield a crude brown solid. FC (DCM/ MeOH: 99/1 to 97/3) afforded the title compound as a pink solid (40 mg, 23%). LC-MS: tR = 0.89 min; [M+H]* = 288.99. 15 b) 2-lodo-benzofuran-4-carboxylic acid [3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide A solution of 2-iodo-benzofuran-4-carboxylic acid (29.5 mg), TBTU (34 mg 1 eq), DIPEA (0.087 mL, 5 eq) in dry DMF (0.325 mL) was stirred at rt for 15 min. Then, was added (4-Aminomethyl-thiazolidin-3-yl)-(2-methyl-5-m-tolyl-thiazol-4-yl)-methanone (34 20 mg, 1.0 eq.) in dry DMF (0.325 mL). Stirring was continued over night. The reaction mixture was partitioned between sat. aq. NaHCO 3 solution/ EtOAc. The organic phase was washed with brine, dried (MgSO 4 ), filtered and concentrated to give 2-iodo benzofuran-4-carboxylic acid [3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4 ylmethyl]-amide (57 mg, 93%) which was used for the next step without further 25 purification. LC-MS: tR = 0.89 min; [M+H]* = 288.99. c) 2-Trifluoromethyl-benzofuran-4-carboxylic acid [3-(2-methyl-5-m-tolyl-thiazole 4-carbonyl)-thiazolidin-4-ylmethyl]-amide A mixture of 2-iodo-benzofuran-4-carboxylic acid [3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide (57 mg), copper (1) iodide (91 mg, 5 eq), methyl 30 2,2-difluoro-2-(fluorosulfonyl)acetate (0.08 mL, 6.5 eq), HMPA (0.17 mL, 10 eq) in dry DMF (2.5 mL) was stirred at 800C for 16 hours under nitrogen. After cooling to rt, the reaction mixture was partitioned between water and EtOAc, the organic phase was washed again with water, dried (MgSO 4 ), filtered and concentrated in vacuo to yield a WO 2008/117241 PCT/IB2008/051110 69 crude yellow-orange oil. FC (Al0 3 , EtOAc/ n-heptane: 7/3) gave the title compound (4.2 mg, 8%) as a white solid. LC-MS: tR = 1 .08 min; [M+H]* = 546.22. II-Biological assays 5 In vitro assay The orexin receptor antagonistic activity of the compounds of formula (1) is determined in accordance with the following experimental method. Experimental method: Intracellular calcium measurements: 10 Chinese hamster ovary (CHO) cells expressing the human orexin-1 receptor and the human orexin-2 receptor, respectively, are grown in culture medium (Ham F-12 with L-Glutamine) containing 300 jig/ml G418, 100 U/ml penicillin, 100 jig/ml streptomycin and 10 % inactivated fetal calf serum (FCS). The cells are seeded at 80'000 cells / well into 96-well black clear bottom sterile plates (Costar) which have been precoated with 15 1% gelatine in Hanks' Balanced Salt Solution (HBSS). All reagents are from Gibco BRL. The seeded plates are incubated overnight at 370C in 5% C02. Human orexin-A as an agonist is prepared as 1 mM stock solution in methanol: water (1:1), diluted in HBSS containing 0.1 % bovine serum albumin (BSA) and 2 mM HEPES for use in the assay at a final concentration of 10 nM. 20 Antagonists are prepared as 10 mM stock solution in DMSO, then diluted in 96-well plates, first in DMSO, then in HBSS containing 0.1 % bovine serum albumin (BSA) and 2 mM HEPES. On the day of the assay, 100 jIl of loading medium (HBSS containing 1% FCS, 2 mM HEPES, 5 mM probenecid (Sigma) and 3 jiM of the fluorescent calcium indicator fluo-3 25 AM (1 mM stock solution in DMSO with 10% pluronic acid) (Molecular Probes) is added to each well. The 96-well plates are incubated for 60 min at 370 C in 5% C02. The loading solution is then aspirated and cells are washed 3 times with 200 jIl HBSS containing 2.5 mM probenecid, 0.1% BSA, 2 mM HEPES. 100 jIl of that same buffer is left in each well.
    WO 2008/117241 PCT/IB2008/051110 70 Within the Fluorescent Imaging Plate Reader (FLIPR, Molecular Devices), antagonists are added to the plate in a volume of 50 1tl, incubated for 20 min and finally 100 I] of agonist is added. Fluorescence is measured for each well at 1 second intervals, and the height of each fluorescence peak is compared to the height of the fluorescence 5 peak induced by 10 nM orexin-A with buffer in place of antagonist. For each antagonist, IC50 value (the concentration of compound needed to inhibit 50 % of the agonistic response) is determined. Antagonistic activities of compounds are in the nanomolar range below 1000 nM with respect to the OX, and/or the OX 2 receptor. Antagonistic activities (IC50 values) of 162 exemplified compounds are in the range of 0.9 - 7245 nM 10 with an average of 181 nM with respect to the OX1 receptor. IC50 values of 164 exemplified compounds are in the range of 0.7-1285 nM with an average of 96 nM with respect to the OX2 receptor. Antagonistic activities of selected compounds are displayed in Table 2. Table 2 Compound OX 1
    IC
    50
    OX
    2
    IC
    50 Compound OX 1
    IC
    50
    OX
    2
    IC
    50 of Example (nM) (nM) of Example (nM) (nM) 4 3 8 110 99 804 11 1 1 112 6 17 31 6 7 119 2 3 38 59 80 127 2 1 41 7 11 132 5 6 79 23 9 133 8 3 83 6 6 141 10 4 91 5946 22 147 9 10 15
    权利要求:
    Claims (13)
    [1] 1. A compound of formula (1) S : H <N N R A O (1) 5 wherein A represents N S R 2 /< R D D ,or D X represents 0, or S; R 2 represents (C_4)alkyl; 10 D represents aryl, which is unsubstituted, mono-, di, or tri-substituted wherein the substituents are independently selected from the group consisting of (C_4)alkyl, (C_4)alkoxy, trifluoromethyl, and halogen; R 1 represents aryl, wherein the aryl group is selected from the group consisting of a phenyl-, a naphthyl-, a 2,3-dihydro-benzofuranyl-, a benzo[1,3]dioxolyl-, a 2,3-dihydro 15 benzo[1,4]dioxinyl-, a 4H-benzo[1,3]dioxinyl, a 2H-chromenyl-, a chromanyl-, a 3,4 dihydro-2H-benzo[1,4]oxazinyl-, and a 3-biphenyl group, wherein said groups are unsubstituted, mono-, di, or tri-substituted wherein the substituents are independently selected from the group consisting of (C_4)alkyl, (C_4)alkoxy, trifluoromethyl, halogen and nitro; 20 or R 1 represents heteroaryl, which is unsubstituted, mono-, di, or tri-substituted, wherein the substituents are independently selected from the group consisting of (C, 4 )alkyl, (C_4)alkoxy, halogen, hydroxy-(C_4)alkyl, and trifluoromethyl; or a pharmaceutically acceptable salt thereof.
    [2] 2. A compound of formula (1) according to claim 1, wherein the stereogenic center at 25 the thiazolidine ring is in (R)-configuration.
    [3] 3. A compound according to claims 1 or 2, wherein A represents WO 2008/117241 PCT/IB2008/051110 72 _ N S R 2 R2 X Dor N or a pharmaceutically acceptable salt thereof.
    [4] 4. A compound according to any one of claims 1 to 3, wherein X represents S; or a pharmaceutically acceptable salt thereof.
    [5] 5 5. A compound according to claims 1 or 2, wherein A represents D or a pharmaceutically acceptable salt thereof.
    [6] 6. A compound according to any one of claims 1 to 5, wherein D represents unsubstituted, mono-, di-, or tri-substituted phenyl, wherein the substituents are 10 independently selected from the group consisting of (C_4)alkyl, (C_4)alkoxy, trifluoromethyl, and halogen; or a pharmaceutically acceptable salt thereof.
    [7] 7. A compound according to any one of claims 1 to 6, wherein R 1 represents aryl, wherein the aryl group is selected from the group consisting of a 15 2,3-dihydro-benzofuranyl-, a benzo[1,3]dioxolyl-, a 2,3-dihydro-benzo[1,4]dioxinyl-, a 4H-benzo[1,3]dioxinyl, a 2H-chromenyl-, a chromanyl-, and a 3,4-dihydro-2H benzo[1,4]oxazinyl group, wherein said groups are unsubstituted, mono-, or di substituted wherein the substituents are independently selected from the group consisting of (C_4)alkyl, (C_4)alkoxy and halogen; 20 or R 1 represents heteroaryl, which is unsubstituted, mono-, di, or tri-substituted, wherein the substituents are independently selected from the group consisting of (C, 4 )alkyl, (C_4)alkoxy, halogen, hydroxy-(C_4)alkyl, and trifluoromethyl; or a pharmaceutically acceptable salt thereof.
    [8] 8. A compound according to any one of claims 1 to 7, wherein 25 R 1 represents heteroaryl, wherein said hetereroaryl is selected from the group consisting of thienyl, thiazolyl, pyrazolyl, pyridyl, indolyl, benzofuranyl, indazolyl, benzoxazolyl, benzisoxazolyl, benzothiazolyl, benzoxadiazolyl, benzothiadiazolyl, imidazo[1,2-a]pyridyl, imidazo[2,1-b]thiazolyl, benzoisothiazolyl, and pyrrolo[2,1- WO 2008/117241 PCT/IB2008/051110 73 b]thiazolyl, wherein said groups are unsubstituted, mono-, di-, or tri-substituted, wherein the substituents are independently selected from the group consisting of (C_4)alkyl, (C_4)alkoxy, halogen, hydroxy-(C_4)alkyl, and trifluoromethyl; or a pharmaceutically acceptable salt thereof. 5
    [9] 9. A compound according to any one of claims 1 to 7, wherein R 1 represents aryl, wherein the aryl group is selected from the group consisting of a 2,3-dihydro-benzofuranyl-, a benzo[1,3]dioxolyl-, a 2,3-dihydro-benzo[1,4]dioxinyl-, a 4H-benzo[1,3]dioxinyl, a 2H-chromenyl-, a chromanyl-, and a 3,4-dihydro-2H benzo[1,4]oxazinyl group, wherein said groups are unsubstituted, mono-, or di 10 substituted wherein the substituents are independently selected from the group consisting of (C_4)alkyl, (C_4)alkoxy and halogen; or a pharmaceutically acceptable salt thereof.
    [10] 10. A compound according to claim 1 selected from the group consisting of: Benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4-carbonyl] 15 thiazolidin-4-ylmethyl}-amide; Benzofuran-4-carboxylic acid {(R)-3-[5-(2-fluoro-phenyl)-2-methyl-thiazole-4-carbonyl] thiazolidin-4-ylmethyl}-amide; Benzofuran-4-carboxylic acid {(R)-3-[5-(3-methoxy-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; 20 Benzofuran-4-carboxylic acid {(R)-3-[2-methyl-5-(3-trifluoromethyl-phenyl)-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; Benzofuran-4-carboxylic acid {(R)-3-[5-(3,4-difluoro-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; Benzofuran-4-carboxylic acid {(R)-3-[5-(2-methoxy-phenyl)-2-methyl-thiazole-4 25 carbonyl]-thiazolidin-4-ylmethyl}-amide; Benzofuran-4-carboxylic acid {(R)-3-[2-methyl-5-(3-trifluoromethyl-phenyl)-oxazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; Benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-p-tolyl-oxazole-4-carbonyl)-thiazolidin 4-ylmethyl]-amide; 30 Benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-oxazole-4-carbonyl] thiazolidin-4-ylmethyl}-amide; Benzofuran-4-carboxylic acid [(R)-3-(2-methyl-4-p-tolyl-thiazole-5-carbonyl)-thiazolidin 4-ylmethyl]-amide; WO 2008/117241 PCT/1B2008/051 110 74 Benzofu ran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-oxazole-4-carbonyl)-th jazolidin 4-yl methyl] -am ide; Benzofuran-4-carboxylic acid {(R)-3-[5-(3,4-dichloro-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; 5 Benzofu ran-4-carboxylic acid {(R)-3-[5-(4-methoxy-phenyl)-2-methyl-oxazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; Benzofuran-4-carboxylic acid [(R)-3-(4'-methyl-biphenyl-2-carbonyl)-thiazolidin-4 ylmethyl]-amide; Benzofuran-4-carboxylic acid [(R)-3-(3',4'-dimethyl-biphenyl-2-carbonyl)-thiazolidin-4 10 ylmethyl]-amide; Benzofuran-4-carboxylic acid [(R)-3-(3'-methyl-biphenyl-2-carbonyl)-thiazolidin-4 ylmethyl]-amide; Benzofu ran-4-carboxylic acid [(R)-3-(3'-methoxy-biphenyl-2-carbonyl)-th iazolidin-4 ylmethyl]-amide; 15 Benzofuran-4-carboxylic acid [(R)-3-(4'-fluoro-biphenyl-2-carbonyl)-thiazolidin-4 ylmethyl]-amide; Benzofu ran-4-carboxylic acid [(R)-3-(4'-methoxy-biphenyl-2-carbonyl)-th iazolidin-4 ylmethyl]-amide; 2,3-Dihydro-benzo[1 ,4]dioxine-5-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl 20 thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 1 -Methyl-i H-indazole-3-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; Benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-phenyl-thiazole-4-carbonyl)-thiazolidin 4-yl methyl] -am ide; 25 1 -Methyl-i H-indazole-3-carboxylic acid [(R)-3-(2-methyl-5-phenyl-thiazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; 1 -Methyl-i H-indazole-3-carboxylic acid [(R)-3-(2-methyl-5-p-tolyl-thiazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; 1 -Methyl-i H-indazole-3-carboxylic acid {(R)-3-[2-methyl-5-(4-trifluoromethyl-phenyl) 30 thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 1 -Methyl-i H-indazole-3-carboxylic acid {(R)-3-[5-(3-fluoro-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; 1 -Methyl-i H-indazole-3-carboxylic acid {(R)-3-[5-(3,4-dimethyl-phenyl)-2-methyl thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; WO 2008/117241 PCT/1B2008/051 110 75 1 -Methyl-i H-indazole-3-carboxylic acid {(R)-3-[5-(2-fluoro-phenyl)-2-methyl-thiazole-4 carbonyl]-th iazolIid in-4-yl methyl}-am ide; 1 -Methyl-i H-indazole-3-carboxylic acid {(R)-3-[5-(3-methoxy-phenyl)-2-methyl-thiazole 4-carbo nyl]-th iazolIidi n-4-yl methyl}-am ide; 5 1 -Methyl-i H-indazole-3-carboxylic acid {(R)-3-[2-methyl-5-(3-trifluoromethyl-phenyl) th iazo le-4-carbo nyl]-th iazol idi n-4-yl methyl}-am ide; i -Methyl-i H-indazole-3-carboxylic acid {(R)-3-[5-(3,4-difluoro-phenyl)-2-methyl th iazo le-4-carbo nyl]-th iazol idi n-4-yl methyl}-am ide; Benzofu ran-4-carboxylic acid [(R)-3-(2-methyl-5-p-tolyl-thiazole-4-carbonyl)-thiazolidin i10 4-yl methyl] -am ide; i -Methyl-i H-indazole-3-carboxylic acid {(R)-3-[5-(2-methoxy-phenyl)-2-methyl-thiazole 4-carbonyl]-thiazolidin-4-ylmethyl}-amide; i -Methyl-i H-indazole-3-carboxylic acid {(R)-3-[2-methyl-5-(3-trifluoromethyl-phenyl) oxazole-4-carbonyl]-thiazol idin-4-ylmethyl}-amide; i5 i -Methyl-i H-indazole-3-carboxylic acid [(R)-3-(2-methyl-5-p-tolyl-oxazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; i -Methyl-i H-indazole-3-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-oxazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; i -Methyl-i H-indazole-3-carboxylic acid [(R)-3-(2-methyl-4-p-tolyl-thiazole-5-carbonyl) 20 thiazolidin-4-ylmethyl]-amide; i -Methyl-i H-indazole-3-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-oxazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; i -Methyl-i H-indazole-3-carboxylic acid {(R)-3-[5-(3,4-dichloro-phenyl)-2-methyl thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 25 i -Methyl-i H-indazole-3-carboxylic acid {(R)-3-[5-(4-methoxy-phenyl)-2-methyl-oxazole 4-carbonyl]-thiazolidin-4-ylmethyl}-amide; i -Methyl-i H-indazole-3-carboxylic acid [(R)-3-(4'-methyl-biphenyl-2-carbonyl) thiazolidin-4-ylmethyl]-amide; i -Methyl-i H-indazole-3-carboxylic acid [(R)-3-(3',4'-dimethyl-biphenyl-2-carbonyl) 30 thiazolidin-4-ylmethyl]-amide; Benzofu ran-4-carboxylic acid {(R)-3-[2-methyl-5-(4-trifluoromethyl-phenyl)-th iazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; i -Methyl-i H-indazole-3-carboxylic acid [(R)-3-(3'-methyl-biphenyl-2-carbonyl) thiazolidin-4-ylmethyl]-amide; WO 2008/117241 PCT/1B2008/051 110 76 1 -Methyl-i H-indazole-3-carboxylic acid [(R)-3-(3'-methoxy-biphenyl-2-carbonyl) th iazolIidi n-4-yl methyl]-am ide; 1 -Methyl-i H-indazole-3-carboxylic acid [(R)-3-(4'-fluoro-biphenyl-2-carbonyl) th iazolIidi n-4-yl methyl]-am ide; 5 1 -Methyl-i H-indazole-3-carboxylic acid [(R)-3-(4'-methoxy-biphenyl-2-carbonyl) th iazolIidi n-4-yl methyl]-am ide; 3- Bro mo-N-{(R) -3- [5-(4-fluo ro-ph enyl)-2-methyl-th iazole-4-carbonyl]-th iazol idi n-4 ylmethyl}-benzamide; i -Methyl-i H-indole-2-carboxylic acid {(R) -3- [5-(4-flu oro-phe nyl)-2-m ethyl -th iazole-4 10 carbonyl]-th iazolIid in-4-yl methyl}-am ide; 4-Bromo-thiophene-2-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; 6-Bromo-pyridine-2-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; 15 2,3-Dihydro-benzo[i ,4]dioxine-5-carboxylic acid [(R)-3-(biphenyl-2-carbonyl) thiazolidin-4-ylmethyl]-amide; Benzofu ran-4-carboxylic acid [(R)-3-(biphenyl-2-carbonyl)-thiazol idin-4-ylmethyl] amide; Benzofu ran-4-carboxylic acid {( R)-3-[5-(3-fluoro-phenyl)-2-methyl-thiazole-4-carbonyl] 20 thiazolidin-4-ylmethyl}-amide; N-[(R)-3-(Biphenyl-2-carbonyl)-thiazolidin-4-ylmethyl]-3-bromo-benzamide; i -Methyl-i H-indole-2-carboxylic acid [(R)-3-(biphenyl-2-carbonyl)-thiazolidin-4 ylmethyl]-amide; i -Methyl-i H-indazole-3-carboxylic acid [(R)-3-(biphenyl-2-carbonyl)-thiazolidin-4 25 ylmethyl]-amide; 4-Bromo-thiophene-2-carboxylic acid [(R)-3-(biphenyl-2-carbonyl)-thiazolidin-4 ylmethyl]-amide; 6-Bromo-pyridine-2-carboxylic acid [(R)-3-(biphenyl-2-carbonyl)-thiazolidin-4-ylmethyl] amide; 30 Benzofuran-4-carboxylic acid {(R)-3-[5-(3,4-dimethyl-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; Naphthalene-i -carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; WO 2008/117241 PCT/1B2008/051 110 77 5-tert- Butyl-2-m ethyl-2 H-pyrazole-3-carboxyl ic acid {(R)-3-[5- (4-f I uo ro-phe nyl)-2 methyl-th iazole-4-carbonyl]-th iazoIid in-4-yl methyl} -am ide; 2,3-Dihydro-benzofuran-7-carboxylic acid {(R)-3[5-(4-fluoro-phenyl)-2-methyl-thiazole 4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 5 2,4-Dimethyl-thiazole-5-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; N-{(R) -3- [5-(4-flu oro-phe nyl)-2-m ethyl -th iazo le-4-carbo nyl]-th iazol idi n-4-yl methyll-3 nitro-benzamide; Benzo[b]thiophene-2-carboxylic acid {(R) -3- [5-(4-flu oro-phe nyl)-2-m ethyl -th iazole-4 10 carbonyl]-thiazolidin-4-ylmethyl}-amide; 2- Methyl -be nzof u ran -4-carboxyl ic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; 2- Methyl -be nzooxazolIe-4-carboxyl ic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 15 Imidazo[1 ,2-a]pyridine-3-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; 2-Methyl-imidazo[1 ,2-a]pyridine-3-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 2,3-Dimethyl-benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 20 carbonyl)-thiazolidin-4-ylmethyl]-amide; 3,4-Dihydro-2H-benzo[1 ,4]oxazine-5-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole 4-carbonyl)-thiazolidin-4-ylmethyl]-amide; 4-Methyl-3,4-Dihydro-2H-benzo[1 ,4]oxazine-5-carboxylic acid [(R)-3-(2-methyl-5-m tolyl-thiazole-4-carbonyl)-thiazolidin-4-ylmethyl]-amide; 25 Chroman-5-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4 ylmethyl]-amide; Chroman-8-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl)-thiazolidin-4 ylmethyl]-amide; 3,4-Dihydro-2H-benzo[1 ,4]oxazine-8-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole 30 4-carbonyl)-thiazolidin-4-ylmethyl]-amide; 4-Methyl-3,4-Dihydro-2H-benzo[1 ,4]oxazine-8-carboxylic acid [(R)-3-(2-methyl-5-m tolyl-thiazole-4-carbonyl)-thiazolidin-4-ylmethyl]-amide; Benzo[d]isoxazole-3-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-th iazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; WO 2008/117241 PCT/1B2008/051 110 78 Benzo[d]isothiazole-3-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; 1-Methyl-i H-indazole-3-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-th iazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; 5 2,3-0 ihydro-benzofu ran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 2,3-Dihydro-benzo[1 ,4]dioxine-5-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 2-Methyl-be nzof u ran -4-carboxyl ic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-th iazole-4 10 carbonyl]-thiazolidin-4-ylmethyl}-amide; Imidazo[1 ,2-a]pyridine-3-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; 2-Methyl-imidazo[1 ,2-a]pyridine-3-carboxylic acid {(R)-3-[5-(4-flIu oro-phe nyl)-2-m ethyl thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 15 2,3-Dimethyl-benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 3,4-Dihydro-2H-benzo[1 ,4]oxazine-5-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2 methyl-th iazole-4-carbonyl]-th iazolidin-4-ylmethyl}-amide; 4-Methyl-3,4-dihydro-2H-benzo[1 ,4]oxazine-5-carboxylic acid {(R)-3-[5-(4-fluoro 20 phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; Chroman-5-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4-carbonyl] thiazolidin-4-ylmethyl}-amide; Chroman-8-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4-carbonyl] thiazolidin-4-ylmethyl}-amide; 25 3,4-Dihydro-2H-benzo[1 ,4]oxazine-8-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2 methyl-th iazole-4-carbonyl]-th iazolidin-4-ylmethyl}-amide; 4-Methyl-3,4-dihydro-2H-benzo[1 ,4]oxazine-8-carboxylic acid {(R)-3-[5-(4-fluoro phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 2,3-Dihydro-benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole 30 4-carbonyl]-thiazolidin-4-ylmethyl}-amide; Benzooxazole-7-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-th iazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; Benzooxazole-7-carboxylic acid {(R)-3-[5-(4-fI uoro-phenyl)-2-methyl-th iazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; WO 2008/117241 PCT/1B2008/051 110 79 2- Methyl -be nzooxazolIe- 7-carboxyl ic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-th iazol idi n-4-yl methyl]-am ide; 2-Methyl-benzooxazole-7-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-th jazole 4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 5 Benzoth iazole-7-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-th iazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; Benzothiazole-7-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; 7-Oh Ioro-benzof uran -4-carboxyl ic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl) 10 thiazolidin-4-ylmethyl]-amide; 7-Ch loro-benzof uran -4-carboxyl ic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; 7-Fluoro-benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; 15 7-Fluoro-benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; Pyrrolo[2,1 -b]thiazole-7-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; Pyrrolo[2,1 -b]thiazole-7-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4 20 carbonyl]-thiazolidin-4-ylmethyl}-amide; 6-Methyl-pyrrolo[2, 1-b]th iazole-7-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-th iazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 6-Methyl-pyrrolo[2, 1-b]thiazole-7-carboxylic acid {(R)-3-[5-(4-flIu oro-phe nyl)-2-m ethyl thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 25 7-Chloro-2-methoxy-2,3-dihydro-benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-m tolyl-thiazole-4-carbonyl)-thiazolidin-4-ylmethyl]-amide; 7-Oh Ioro-2-methoxy-2,3-di hydro-benzof uran -4-carboxyl ic acid {( R)-3-[5-(4-fI uoro phenyl)-2-methyl-thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 2-Oh Ioro-benzothiazole-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 30 carbonyl)-thiazolidin-4-ylmethyl]-amide; 2-Ohloro-benzothiazole-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole 4-carbonyl]-thiazolidin-4-ylmethyl}-amide; Benzoth iazole-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-th iazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; WO 2008/117241 PCT/IB2008/051110 80 Benzoth iazole-4-carboxylic acid {(R)-3-[5-(4-fI uoro-phenyl)-2-methyl-th iazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; Benzo[2,1,3]th iadiazole-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 5 Benzo[2,1,3]thiadiazole-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole 4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 7-Trifl uoromethyl-benzofu ran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 7-Trifl uoromethyl-benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl 10 thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 3-Methyl-benzofu ran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; 3-Methyl-benzofu ran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole-4 carbonyl]-thiazolidin-4-ylmethyl}-amide; 15 Benzo[2,1,3]oxadiazole-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; Benzo[2,1,3]oxadiazole-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl-thiazole 4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 2-Hydroxymethyl-benzofu ran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 20 carbonyl)-thiazolidin-4-ylmethyl]-amide; 2-Hydroxymethyl-benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 2-Methyl-benzofu ran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4-carbonyl) thiazolidin-4-ylmethyl]-amide; 25 5-Chloro-2-methyl-benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 7-Chloro-2-methyl-benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 7-Fluoro-2-methyl-benzofu ran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 30 carbonyl)-thiazolidin-4-ylmethyl]-amide; 2-Methyl-7-trifluoromethyl-benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl thiazole-4-carbonyl)-thiazolidin-4-ylmethyl]-amide; 6-Chloro-2-methyl-benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; WO 2008/117241 PCT/IB2008/051110 81 6-Fluoro-2-methyl-benzofu ran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 2-Methyl-6-trifluoromethyl-benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl thiazole-4-carbonyl)-thiazolidin-4-ylmethyl]-amide; 5 5-Chloro-2-methyl-benzofuran-4-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 7-Chloro-2-methyl-benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 7-Fluoro-2-methyl-benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl 10 thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 2-Methyl-7-trifluoromethyl-benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2 methyl-thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 6-Chloro-2-methyl-benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 15 6-Fluoro-2-methyl-benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 2-Methyl-6-trifluoromethyl-benzofuran-4-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2 methyl-thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 2-Trifl uoromethyl-benzofu ran-4-carboxylic acid [3-(2-methyl-5-m-tolyl-thiazole-4 20 carbonyl)-thiazolidin-4-ylmethyl]-amide; 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 6-Methyl-imidazo[2,1-b]thiazole-5-carboxylic acid [(R)-3-(2-methyl-5-phenyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 25 6-Methyl-imidazo[2,1-b]thiazole-5-carboxylic acid [(R)-3-(2-methyl-5-p-tolyl-thiazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid {(R)-3-[2-methyl-5-(4-trifluoromethyl phenyl)-thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid {(R)-3-[5-(3-fluoro-phenyl)-2-methyl 30 thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid {(R)-3-[5-(3,4-dimethyl-phenyl)-2 methyl-thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid {(R)-3-[5-(2-fluoro-phenyl)-2-methyl thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; WO 2008/117241 PCT/IB2008/051110 82 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid {(R)-3-[5-(3-methoxy-phenyl)-2 methyl-th iazole-4-carbonyl]-th iazolidin-4-ylmethyl}-amide; 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid {(R)-3-[2-methyl-5-(3-trifluoromethyl phenyl)-thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 5 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid {(R)-3-[5-(3,4-difluoro-phenyl)-2 methyl-th iazole-4-carbonyl]-th iazolidin-4-ylmethyl}-amide; 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid {(R)-3-[5-(2-methoxy-phenyl)-2 methyl-th iazole-4-carbonyl]-th iazolidin-4-ylmethyl}-amide; 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid {(R)-3-[2-methyl-5-(3-trifluoromethyl 10 phenyl)-oxazole-4-carbonyl]-thiazolidin-4-ylmethyl}-am ide; 6-Methyl-imidazo[2,1-b]thiazole-5-carboxylic acid [(R)-3-(2-methyl-5-p-tolyl-oxazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid {(R)-3-[5-(4-fluoro-phenyl)-2-methyl oxazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 15 6-Methyl-imidazo[2,1-b]thiazole-5-carboxylic acid [(R)-3-(2-methyl-4-p-tolyl-thiazole-5 carbonyl)-thiazolidin-4-ylmethyl]-amide; 6-Methyl-imidazo[2,1-b]thiazole-5-carboxylic acid [(R)-3-(2-methyl-5-m-tolyl-oxazole-4 carbonyl)-thiazolidin-4-ylmethyl]-amide; 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid {(R)-3-[5-(3,4-dichloro-phenyl)-2 20 methyl-thiazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid {(R)-3-[5-(4-methoxy-phenyl)-2 methyl-oxazole-4-carbonyl]-thiazolidin-4-ylmethyl}-amide; 6-Methyl-imidazo[2,1-b]thiazole-5-carboxylic acid [(R)-3-(4'-methyl-biphenyl-2 carbonyl)-thiazolidin-4-ylmethyl]-amide; 25 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid [(R)-3-(3',4'-dimethyl-biphenyl-2 carbonyl)-thiazolidin-4-ylmethyl]-amide; 6-Methyl-imidazo[2,1-b]thiazole-5-carboxylic acid [(R)-3-(3'-methyl-biphenyl-2 carbonyl)-thiazolidin-4-ylmethyl]-amide; 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid [(R)-3-(3'-methoxy-biphenyl-2 30 carbonyl)-thiazolidin-4-ylmethyl]-amide; 6-Methyl-imidazo[2,1-b]thiazole-5-carboxylic acid [(R)-3-(4'-fluoro-biphenyl-2-carbonyl) thiazolidin-4-ylmethyl]-amide; 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid [(R)-3-(4'-methoxy-biphenyl-2 carbonyl)-thiazolidin-4-ylmethyl]-amide; and WO 2008/117241 PCT/IB2008/051110 83 6-Methyl-imidazo[2,1 -b]thiazole-5-carboxylic acid [(R)-3-(biphenyl-2-carbonyl) thiazolidin-4-ylmethyl]-amide; or a pharmaceutically acceptable salt of such a compound.
    [11] 11. A compound of any one of claims 1 to 10, or a pharmaceutically acceptable salt 5 thereof, for use as medicament.
    [12] 12. Use of a compound according to any of claims 1 to 10, or of a pharmaceutically acceptable salt thereof, for the preparation of a medicament for the prevention or treatment of diseases selected from the group consisting of dysthymic disorders including major depression and cyclothymia, affective neurosis, all types of manic 10 depressive disorders, delirium, psychotic disorders, schizophrenia, catatonic schizophrenia, delusional paranoia, adjustment disorders and all clusters of personality disorders; schizoaffective disorders; anxiety disorders including generalized anxiety, obsessive compulsive disorder, posttraumatic stress disorder, panic attacks, all types of phobic anxiety and avoidance; separation anxiety; all psychoactive substance use, 15 abuse, seeking and reinstatement; all types of psychological or physical addictions, dissociative disorders including multiple personality syndromes and psychogenic amnesias; sexual and reproductive dysfunction; psychosexual dysfunction and addiction; tolerance to narcotics or withdrawal from narcotics; increased anaesthetic risk, anaesthetic responsiveness; hypothalamic-adrenal dysfunctions; disturbed 20 biological and circadian rhythms; sleep disturbances associated with diseases such as neurological disorders including neuropathic pain and restless leg syndrome; sleep apnea; narcolepsy; chronic fatigue syndrome; insomnias related to psychiatric disorders; all types of idiopathic insomnias and parasomnias; sleep-wake schedule disorders including jet-lag; all dementias and cognitive dysfunctions in the healthy 25 population and in psychiatric and neurological disorders; mental dysfunctions of aging; all types of amnesia; severe mental retardation; dyskinesias and muscular diseases; muscle spasticity, tremors, movement disorders; spontaneous and medication-induced dyskinesias; neurodegenerative disorders including Huntington's, Creutzfeld-Jacob's, Alzheimer's diseases and Tourette syndrome; Amyotrophic lateral sclerosis; 30 Parkinson's disease; Cushing's syndrome; traumatic lesions; spinal cord trauma; head trauma; perinatal hypoxia; hearing loss; tinnitus; demyelinating diseases; spinal and cranial nerve diseases; ocular damage; retinopathy; epilepsy; seizure disorders; absence seizures, complex partial and generalized seizures; Lennox-Gastaut syndrome; migraine and headache; pain disorders; anaesthesia and analgesia; WO 2008/117241 PCT/IB2008/051110 84 enhanced or exaggerated sensitivity to pain such as hyperalgesia, causalgia, and allodynia; acute pain; burn pain; atypical facial pain; neuropathic pain; back pain; complex regional pain syndrome I and II; arthritic pain; sports injury pain; dental pain; pain related to infection e.g. by HIV; post-chemotherapy pain; post-stroke pain; post 5 operative pain; neuralgia; osteoarthritis; conditions associated with visceral pain such as irritable bowel syndrome; eating disorders; diabetes; toxic and dysmetabolic disorders including cerebral anoxia, diabetic neuropathies and alcoholism; appetite, taste, eating, or drinking disorders; somatoform disorders including hypochondriasis; vomiting/nausea; emesis; gastric dyskinesia; gastric ulcers; Kallman's syndrome 10 (anosmia); impaired glucose tolerance; intestinal motility dyskinesias; hypothalamic diseases; hypophysis diseases; hyperthermia syndromes, pyrexia, febrile seizures, idiopathic growth deficiency; dwarfism; gigantism; acromegaly; basophil adenoma; prolactinoma; hyperprolactinemia; brain tumors, adenomas; benign prostatic hypertrophy, prostate cancer; endometrial, breast, colon cancer; all types of testicular 15 dysfunctions, fertility control; reproductive hormone abnormalities; hot flashes; hypothalamic hypogonadism, functional or psychogenic amenorrhea; urinary bladder incontinence; asthma; allergies; all types of dermatitis, acne and cysts, sebaceous gland dysfunctions; cardiovascular disorders; heart and lung diseases, acute and congestive heart failure; hypotension; hypertension; dyslipidemias, hyperlipidemias, 20 insulin resistance; urinary retention; osteoporosis; angina pectoris; myocardial infarction; arrhythmias, coronary diseases, left ventricular hypertrophy; ischemic or haemorrhagic stroke; all types of cerebrovascular disorders including subarachnoid haemorrhage, ischemic and hemorrhagic stroke and vascular dementia; chronic renal failure and other renal diseases; gout; kidney cancer; urinary incontinence; and other 25 diseases related to general orexin system dysfunctions.
    [13] 13. Use of a compound according to any of claims 1 to 10, or of a pharmaceutically acceptable salt thereof, for the preparation of a medicament for the prevention or treatment of diseases selected from the group consisting of all types of sleep disorders, of stress-related syndromes, of psychoactive substance use and abuse, of cognitive 30 dysfunctions in the healthy population and in psychiatric and neurologic disorders, of eating or drinking disorders.
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    法律状态:
    2011-12-01| MK1| Application lapsed section 142(2)(a) - no request for examination in relevant period|
    优先权:
    申请号 | 申请日 | 专利标题
    IB2007051048||2007-03-26||
    IBPCT/IB2007/051048||2007-03-26||
    IB2008050620||2008-02-21||
    IBPCT/IB2008/050620||2008-02-21||
    PCT/IB2008/051110|WO2008117241A2|2007-03-26|2008-03-25|Thiazolidine derivatives as orexin receptor antagonists|
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